LC-MS/MS量化方法对分析结果的影响,以全国性美国环境样本为例
Hansa Y Magee1, Megan M Maurer2, April Cobos2
1Arizona State University, Biodesign Center for Environmental Health Engineering, Tempe, AZ, USA; Arizona State University, Department of Biomedical Informatics, College of Health Solutions, Tempe, AZ, USA.
The Science of the total environment
|May 31, 2023
概括
量化方法的选择对环境化学的分析结果产生重大影响. 这项研究表明,同位素与真实类型的稀释是最常见的,但准确性因分析物和矩阵而异.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 量化方法在环境化学中至关重要,但它们对分析结果质量的影响未得到充分研究.
- 常见的方法包括外部校准,同位素稀释 (使用目标或非目标标准) 和标准添加.
- 液体染色学双重质谱法 (LC-MS/MS) 经常用于分析诸如污水污泥等复杂矩阵.
研究的目的:
- 评估和比较四种常见量化方法的性能:外部校准,同位素稀释与目标类似物,同位素稀释与非目标标准,和标准加法.
- 评估这些方法对污水污泥中的抗生素分析结果的影响,使用液态染色学双重质谱法 (LC-MS/MS).
主要方法:
- 四种量化方法的实验室内评估和元分析 (n=66).
- 在美国的污水污泥样本中应用分析抗生素的方法,使用LC-MS/MS.
- 将结果与基准方法进行比较,以确定过高/低估和识别矩阵效应.
主要成果:
- 根据方法和分析剂观察到显著的高估值 (110-450%) 和低估值 (10-60%),使用外部校准作为红红的基准.
- 作为基准的标准加值显示了更广泛的变化 (高估101-14,700%,低估6-98%).
- 矩阵效应,例如对红红素的信号抑制,是依赖于分析剂和矩阵的.
结论:
- 量化方法的选择极大地影响了环境化学数据的可靠性.
- 由于缺少同位素标记的类似物,需要实验确定以确定给定分析物的最准确方法.
- 已发表的文献显示,人们更喜欢用真实目标类型的同位素稀释,但这可能并不总是最准确的方法.
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