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集成的lncRNA转录组学,蛋白组学和代谢组学,以识别脱氧尼瓦伦醇治疗的IPEC-J2细胞中的早期细胞响应变异

Andong Zha¹, Simeng Liao¹, Bie Tan²

¹Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China; University of Chinese Academy of Sciences, Beijing, 100008, China.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|May 31, 2023

概括

脱氧尼瓦伦醇 (DON) 菌毒素污染食品和料,危害人类和牲畜的健康. 这项研究揭示了DON DON.

科学领域:

* 毒理学和分子生物学
* 动物科学和食品安全

背景情况:

* 菌毒素,特别是脱氧尼瓦伦醇 (DON),是食品和料中普遍存在的污染物.
*DON对人类健康和畜牧业生产构成重大风险.
*已知DON会损害肠道上皮质屏障的功能.

研究的目的:

* 阐明DON引起的肠上皮细胞损伤背后的分子机制.
* 调查低剂量 (LDON) 和高剂量 (HDON) DON暴露对猪肠上皮细胞 (IPEC-J2) 的差异影响.

主要方法:

* 用200 ng/ml (LDON) 或2000 ng/ml (HDON) DON化IPEC-J2细胞6小时.
* lncRNA测序,代谢学和蛋白质学的应用.
*对转录组学,蛋白组学和代谢组学数据的综合分析.

主要成果:

* LDON上调调节了核糖体生物发生,结合体,泛胺介导蛋白解和RNA运输,同时降低了代谢途径的调节.
* HDON上调调节了核糖体生物发生和结合酶体,并降调调节了DNA修复途径 (基切除修复,细胞循环,DNA复制,同源重组,不匹配修复).
*与LDON相比,HDON的上调会粘附到结点,Hippo信号通路和癌症中的通路,同时降低DNA复制的调节.
* LDON 和 HDON 治疗都主要降低了不和脂肪酸生物合成和脂肪酸代谢的调节.

结论:

*DON暴露显著改变IPEC-J2细胞中的细胞通路,影响核糖体生物发生,DNA修复和新陈代谢.
*在LDON和HDON之间观察到的不同反应表明DON诱导的肠损伤的剂量依赖机制.
*这些发现为DON对肠道上皮细胞的影响提供了新的见解,有助于制定预防和治疗策略.

关键词:

脱氧尼瓦伦烯 (deoxynivalenol) 是一种除氧化烯 (deoxynivalenol) 的一种.脂肪酸是一种脂肪酸.在IPEC-J2中.肠道上皮质屏障肠道上皮质屏障拼接一些osome.

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