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天然深层欧性溶剂作为Humicola insolens cutinase的热稳定剂

Angela Romano¹, Simona Varriale², Cinzia Pezzella³

¹Department of Civil, Chemical Environmental and Materials Engineering, University of Bologna, via Terracini 28, 40131 Bologna, Italy.

New biotechnology

|May 31, 2023

概括

天然的深溶性溶剂 (NADES) 可以稳定酶. 胆二酸盐和西利醇在1:1的比率下,在20%的重量中,显著提高了90°C的Humicola insolens cutinase (HiC) 稳定性.

科学领域:

生物催化剂是一种生物催化剂.
绿色化学绿色化学
蛋白质稳定蛋白质稳定

背景情况:

深度环氧溶剂 (DES) 是新兴的绿色溶剂.
DES显示出作为生物催化剂媒介的潜力,提高了酶效率.
天然深层环氧溶剂 (NADES) 提供了一个可持续的替代品.

研究的目的:

评估Humicola insolens cutinase (HiC) 在NADESs中的稳定性.
为了确定最佳的NADES成分和度用于酶稳定.
评估NADES对HiC热稳定性的影响.

主要方法:

分子对接模拟以评估结合能量和方向.
使用选定的组件制备NADES水溶液.
在90°C时对NADES中的HiC进行热稳定性测试.

主要成果:

胆二酸盐和糖醇被确定为最佳的NADES成分.
胆二酸:利醇在1:1的比率下,在20重%的度下稳定了HiC.
在优化的NADES中,HiC半衰期在90°C时增加了三倍.

结论:

NADESs可以有效地稳定像HiC这样的酶.
优化的NADES配方可以显著提高酶的热稳定性.
这项研究为在工业生物催化剂中使用NADES提供了基础.

关键词:

胆二酸盐是一种胆二酸盐.酶的稳定性酶的稳定性分子对接是分子对接.这些都是NADES NADES NADES.克西利托尔 (Xylitol) 是一种素.

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