水友互动液态色谱与甲醇-水溶解剂在一个氧化物上
James Otis1, Jörg Hofmann2, Thomas Schmidt3
1University of Toronto, Department of Biochemistry, 1 King's College Circle, Toronto, ONM5G 0A4, Canada.
Analytica chimica acta
|May 31, 2023
概括
齐奥利特微孔通过创建富含水相来增强水友交互色谱 (HILIC) 中的分离. 与传统的HILIC方法相比,这种新的方法为聚醇和单糖分离体提供了更高的选择性.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 材料科学 材料科学 材料科学
背景情况:
- 水友互动色谱 (HILIC) 使用有机溶剂-水混合物和水友静止相来分离分析物.
- 在HILIC中的保留依赖于静止阶段的富含水的层,通过增加有机溶剂分数来增强选择性,但受到分析物溶解度的限制.
- 提高HILIC性能需要开发新的水友静止相.
研究的目的:
- 为了研究Y-zeolite作为HILIC的新型静止相的潜力.
- 为了评估基于Y-泽奥利特的HPLC柱对水友性化合物的分离能力.
- 为了比较Y-化物HILIC的选择性与传统的HILIC方法.
主要方法:
- 一个125毫米的HPLC柱被装满了5微米的Na+形式的Y-化石 (FAU类型).
- 染色分离使用甲醇水混合物作为化剂进行.
- 亚利法醇,多醇和单糖被注射进来,以测试柱子的性能和选择性.
主要成果:
- Y-泽奥利特柱显示了乙烯基醇,甘油,红醇,醇和内醇的保留,保留时间随基组数增加而增加.
- 分离的原因是石微孔中的分区平衡,以及与石表面和Na+离子的选择性相互作用.
- 阿拉比诺斯和果糖被分离成它们的复合体形式,这些形式在更高的温度 (60°C) 上合并.
结论:
- -泽奥利特作为水友静止相起作用,其微孔取代了传统HILIC的静止水层.
- 与传统的HILIC相比,Y-泽奥利特柱对多聚醇和单糖分离体具有更高的选择性.
- 这项研究介绍了Y-zeolite作为先进的HILIC应用的有希望的材料.
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