Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Anoxygenic Photosynthesis01:30

Anoxygenic Photosynthesis

54
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
54
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation02:17

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation

3.4K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.4K
Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

164
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
164
Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide02:44

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

10.4K
Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.
10.4K
Reduction of Alkenes: Catalytic Hydrogenation02:13

Reduction of Alkenes: Catalytic Hydrogenation

12.2K
Alkenes undergo reduction by the addition of molecular hydrogen to give alkanes. Because the process generally occurs in the presence of a transition-metal catalyst, the reaction is called catalytic hydrogenation.
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
12.2K
Catalysis02:50

Catalysis

27.1K
The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
27.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Plug-and-play - enzymatic amino acid production from methanol and carbon dioxide.

Nature communications·2026
Same author

Engineering Access to Stereoirregular Polymer Microstructures Enables Improved Processability of Microbial Poly(3-hydroxybutyrate).

Biomacromolecules·2026
Same author

Synthesis of thermostable artificial nicotinamide cofactors: carba-NAD<sup>+</sup> and carba-NADP<sup></sup>.

RSC chemical biology·2026
Same author

Biochemical and Mechanistic Insights into O<sub>2</sub> Scavenging and Stability of the Soluble Hydrogenase from <i>Hydrogenophaga pseudoflava</i>.

ACS omega·2026
Same author

Hydroxo-bridged active site of flavodiiron NO reductase revealed by NRVS and DFT.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Advances in synthetic methanotrophy for sustainable C1 bioconversion.

Trends in biotechnology·2025

相关实验视频

Updated: Jul 28, 2025

Light-driven Enzymatic Decarboxylation
09:58

Light-driven Enzymatic Decarboxylation

Published on: May 22, 2016

11.8K

没有氧气的基于酶的氧化生物催化剂.

Ammar Al-Shameri1, Dominik L Siebert1, Samuel Sutiono1

  • 1Chair of Chemistry of Biogenic Resources, TUM Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Schulgasse 16, 94315, Straubing, Germany.

Nature communications
|May 31, 2023
PubMed
概括

一个基于酶的新型系统用于尼古丁胺氨酸二核酸 (NAD+) 再生,其性能优于传统的NADH氧化酶 (NOX) 系统. 这种可持续的生物催化剂方法为化学生产提供了更高的效率和技术简单性.

更多相关视频

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.3K
Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells
06:39

Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells

Published on: October 20, 2023

2.9K

相关实验视频

Last Updated: Jul 28, 2025

Light-driven Enzymatic Decarboxylation
09:58

Light-driven Enzymatic Decarboxylation

Published on: May 22, 2016

11.8K
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.3K
Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells
06:39

Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells

Published on: October 20, 2023

2.9K

科学领域:

  • 生物催化剂和可持续化学
  • 辅助因子再生系统 辅助因子再生系统
  • 酶工程是一种酶工程.

背景情况:

  • 生物催化提供了化学合成的清洁途径,通常需要尼古丁胺胺氨基二核酸 (NAD+) 再生.
  • NADH氧化酶 (NOX) 通常用于NAD+再生,但在氧气供应和质量转移方面面临挑战.
  • 有效的NAD+再生对于生物催化过程的经济可行性至关重要.

研究的目的:

  • 引入和评估一种基于使用酶的气演变的新型NAD+再生系统.
  • 为了比较基于酶的系统与传统的NOX系统的性能.
  • 为了证明酶系统在酸生产的多酶级联中的适用性.

主要方法:

  • 通过酶和H2进化进行体外NAD+再生.
  • 将酶系统集成到多酶级联中,用于将糖转化为酸.
  • 对NOX系统的NAD+回收效率和可扩展性的比较分析.

主要成果:

  • 与NOX相比,基于酶的系统实现了显著更高的NAD+循环 (高达44,000mol/mol酶) NOX.
  • 该系统表现出卓越的技术简单性,灵活性和整体输出.
  • 酶系统有效地从糖分中产生酸,只有绿色的H2作为副产品,即使存在氧气.

结论:

  • 基于酶的NAD+再生是一种可扩展和优越的替代NOX用于生物催化.
  • 这种系统提供了提高效率,技术优势和环境效益 (绿色H2副产品).
  • 开发的系统对可持续化学品生产平台具有重大前景.