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相关概念视频

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

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The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
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Forced Transdifferentiation01:28

Forced Transdifferentiation

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Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
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Methods of Nuclear Reprogramming01:24

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Somatic to iPS Cell Reprogramming01:29

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Introduction to Nuclear Reprogramming01:14

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Lineage Commitment01:21

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Commitment is the  process whereby stem cells:
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In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
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在B细胞分化过程中代谢重编程

Sophie Stephenson1, Gina M Doody2

  • 1Division of Haematology and Immunology, Leeds Institute of Medical Research, University of Leeds, Leeds, UK.

Methods in molecular biology (Clifton, N.J.)
|May 31, 2023
PubMed
概括
此摘要是机器生成的。

本研究描述了人类B细胞体外分化的方法,详细介绍了它们转化为抗体分泌等离体细胞期间的代谢转变. 这些方法可以评估B细胞激活和分化阶段的代谢重编程.

关键词:
B细胞是B细胞的组成部分.不同化的差异化细胞等离子体细胞血布拉斯特是一种血.

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科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 代谢研究研究 代谢研究

背景情况:

  • 乙细胞分化是一个复杂的过程,涉及显著的代谢变化.
  • 了解这些代谢变化对于研究B细胞功能和抗体产生至关重要.

研究的目的:

  • 描述人类B细胞体外分化的方法.
  • 为了在体外纳入B细胞分化的过渡阶段.
  • 为了促进在B细胞分化过程中对代谢重编程的评估.

主要方法:

  • 在体外分化人类B细胞.
  • 纳入过渡阶段:休息,激活 (DNA合成/分裂) 和终端分化 (静止/分泌).
  • 在每个分化阶段评估代谢需求.

主要成果:

  • 在体外逐步分化人类B细胞的既定方法.
  • 标志着伴随B细胞激活和血细胞形成的代谢重编程.
  • 提供了一个模型来研究B细胞到血细胞过渡期间的代谢变化.

结论:

  • 描述的体外方法使B细胞代谢重编程的全面研究成为可能.
  • 这些方法对于研究抗体分泌的代谢需求有价值.
  • 进一步的研究可以利用这些模型来探索与B细胞相关的疾病和疗法.