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条形码协会感染:一种可扩展,内部控制的方法来研究宿主细胞结合和病原性细菌的入侵

Maria Letizia Di Martino¹, Mikael E Sellin²

¹Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden. ml.dimartino@imbim.uu.se.

Methods in molecular biology (Clifton, N.J.)

|May 31, 2023

概括

这项研究引入了一种新的高通量方法,用于评估细菌的入侵和与宿主细胞的结合. 这种技术改进了传统的测试,提供了一种更有效和可靠的方法来研究细菌毒性因素.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
细胞生物学细胞生物学

背景情况:

传统的针对细菌宿主细胞入侵的 جنت米辛保护试验是劳动密集型的,容易产生显著的实验变异性.
研究细菌毒性因素,例如那些参与宿主细胞入侵的因素,对于理解病原和开发对策至关重要.

研究的目的:

描述一种新的,内部控制的,中高通量方法,用于量化细菌宿主细胞的结合和入侵.
为在宿主-病原体相互作用中对细菌毒性进行遗传分析提供灵活和广泛适用的工具.

主要方法:

该方法结合了基因标记的同源细菌菌株的联合体与修改的 gentamicin 保护试验.
这种方法允许在单个实验中同时评估多个细菌突变菌株.
该协议适用于体外培养宿主细胞和体外动物模型.

主要成果:

开发的方法可以严格量化不同细菌突变的宿主细胞结合和侵入性质.
与传统方法相比,它显著降低了实验噪声.
不同实验模型之间的可比基因读数可促进跨模型比较.

结论:

这种内部控制的高通量方法为研究细菌宿主细胞相互作用提供了强大的和多功能工具.
它广泛适用于沙门氏菌以外的各种病原体,有助于对毒性因素的比较分析.
该方法简化了对细菌病原学的研究和开发新型治疗策略的研究.

关键词:

有约束力的约束力有遗传标记的菌株.宿主病原体相互作用这是入侵的入侵.沙门氏菌 Typhimurium 是一个类型.

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