相关实验视频

Updated: Jul 28, 2025

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

一种靠近诱导的化反应,由抗体-抗原相互作用与相邻的表皮层触发

Kentaro Nishiyama¹, Hiroki Akiba^1,2, Satoshi Nagata²

¹Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, 606-8501, Japan.

Angewandte Chemie (International ed. in English)

|June 1, 2023

概括

我们开发了一种新型的抗原模拟化学反应 (BATER),它使用抗体加速反应. 这种抗原模板式方法对生物系统中的应用有希望.

科学领域:

化学生物学化学生物学
免疫学免疫学免疫学
生物结合化学生物结合化学

背景情况:

靠近诱导的反应通过模板交互提供了特定位置和速度.
抗体-抗原相互作用以前没有被用来诱导化学反应.
需要新的模板策略来控制化学转换.

研究的目的:

引入一种新型的抗原模拟化学反应 (BATER) 系统.
用抗体-抗原识别来证明化学反应的加速.
建立一种可视化和优化生物应用BATER的方法.

主要方法:

将反应性功能组结合到针对同一抗原的不同表位的两个抗体.
使用化基点击化学来监控和优化BATER过程.
调查链接长度和抗体表位选择对反应动力学的影响.

主要成果:

在特定抗原的存在下,BATER加速化学反应.
反应速率取决于连接抗体的链接器的长度.
抗体表位的选择显著影响了反应速度.
化基点击化学有效地可视化了BATER的进度以进行优化.

结论:

贝皮托普抗原模拟化学反应 (BATER) 是一种可行的近距离诱导反应策略.
抗体-抗原相互作用可以用来模拟和加速化学转变.
"BATER系统显示出在生物系统中各种应用的潜力.

关键词:

抗体是一种抗体.生物结合的生物结合点击化学点击化学 .上层层是什么意思上层是什么意思模板综合模板综合

更多相关视频

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

Published on: September 15, 2016

相关概念视频

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Cross-reactivity

Cross-reactivity