转录学方法揭示了果果中季节性晒伤发展中的动态事件
Jessica M Waite1,2, Elizabeth A Kelly3, Huiting Zhang1,4
1USDA Agricultural Research Service, Tree Fruit Research Laboratory, 1104 N. Western Ave., Wenatchee, WA, 98801, USA.
G3 (Bethesda, Md.)
|June 1, 2023
概括
热应激会影响果的质量,导致晒伤. 这项研究揭示了新的基因和受季初高温影响的途径,有助于开发有弹性的果品种.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 果在高热,高光和低湿度条件下容易受到晒伤和作物损失.
- 以前的研究集中在季后热应激和有限数量的基因上.
- 了解季初的压力反应对于果的弹性至关重要.
研究的目的:
- 调查季初热应激后果的动态基因表达.
- 确定导致太阳热引起的伤害的新型分子机制.
- 为耐热果品种的育种策略提供信息.
主要方法:
- 对中季果进行热应激的现场应用.
- 在压力后的24,48,72小时内进行时间过程组织采集.
- 转录组分析以确定差异表达基因 (DEGs).
主要成果:
- 确定了有限数量的差异表达基因 (DEGs).
- 观察到丰富的功能术语,但很少与以前已知的晒伤途径有关.
- 大多数DEG都是新的,以前没有与果中的晒伤或热应激有关.
结论:
- 季初的热应激会触发果中独特的基因表达模式.
- 除了烯和ROS之外,新的分子通路也参与了热反应.
- 这些发现为培育对环境压力的耐受性提高的果提供了基础.
相关概念视频
Gene Regulation During Sporulation
42
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
42
Fruit Development, Structure, and Function
22.5K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
22.5K
Transcription
147.3K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
147.3K


