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直接电流电场刺激促进了间酶体干细胞的增殖,并保持了干细胞的干性

Mengchang Liu¹, Defu Xie¹, Huizhen Zeng¹

¹Southwest Medical University, No. 1 Section 1, Xianglin Road, Luzhou City, Sichuan Province, 646000, China.

|June 1, 2023

概括

电场 (EFs) 增强了介酶干细胞的增殖. 一个新的生物反应器为脂肪衍生的介质干细胞提供EFs,促进细胞生长,同时保持再生医学应用的干细胞.

科学领域:

再生医学是一种再生医学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

介质细胞干细胞 (MSCs) 对再生医学至关重要.
众所周知,电场 (EF) 影响细胞功能,如增殖和分化.
优化MSC增殖对于组织工程至关重要.

研究的目的:

开发和测试一种新的生物反应器,用于将直流电场应用于体外细胞培养物.
研究EF刺激对脂肪衍生性介质干细胞 (ADMSC) 增殖和干细胞的作用.
为研究EF对干细胞的影响提供一种成本效益和时间效率高的方法.

主要方法:

一个定制的生物反应器被设计为同时向六个细胞培养井应用直流EF.
在体外培养了由脂质衍生的中干细胞,并在生物反应器内暴露于EF刺激.
细胞增殖率和干性标志物被评估后刺激.

主要成果:

在新型生物反应器中用EFs刺激的ADMSC显示出明显增加的增殖率.
EF刺激并没有损害ADMSCs的干性.
生物反应器证明了减少了实验时间和成本效益.

结论:

开发的生物反应器有效地应用EF刺激来增强ADMSC的扩散.
在再生医学中,EF刺激是改善干细胞扩张的有希望的方法.
这项技术为组织工程和推进干细胞研究提供了有价值的工具.

关键词:

脂肪衍生介质干细胞 (Ad-MSCs) 是指脂肪衍生介质干细胞.生物反应器是一个生物反应器.细胞的增殖细胞的增殖.电场刺激的电场刺激.再生医学是一种再生医学.

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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...