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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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相关实验视频

Updated: Jul 28, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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一种用于生成捕获诱的方法,用于有针对性的测序.

Balaji Sundararaman1,2, Alisa O Vershinina3, Samantha Hershauer1,2

  • 1Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, USA.

Nucleic acids research
|June 1, 2023
PubMed
概括
此摘要是机器生成的。

这项研究介绍了循环核酸丰富反应剂 (CNER),这是一种创建DNA探针的新方法. 对于像古代DNA这样具有挑战性的样品,CNER显著改善了针对性的高通量测序.

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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 混合化捕获使成本效益高的高通量向测序成为可能.
  • 它对于分析来自古代,环境或法医样本的低目标含量,碎片化DNA至关重要.
  • 现有的有针对性的方法可能会因为DNA质量受到损害而失败.

研究的目的:

  • 开发一种用于杂交捕获的新型DNA诱合成方法.
  • 引入循环核酸丰富反应剂 (CNER) 方法.
  • 评估CNER的效率与商业方法相比,用于古代DNA测序.

主要方法:

  • CNER利用滚动循环放大,然后进行限制消化,以生产杂交探头.
  • 探针设计用于马基因组中的23771个单核酸多态位点.
  • 将CNER捕获效率与使用十个古老马DNA库的商业方法进行了比较.

主要成果:

  • 对于有针对性的测序,CNER成功生成了杂交探头.
  • 与商业方法 (66.5%) 相比,CNER捕获了更多的目标 (90.5%).
  • 在CNER的研究中,每个样本的平均测序深度更高.

结论:

  • CNER是一种有效和高效的方法,用于在杂交捕获中合成DNA诱.
  • CNER的性能优于商业方法,可以对古代DNA进行有针对性的测序.
  • 这种方法推进了对具有挑战性的DNA样本的基因组分析.