在使用功能性Polycomb响应元件的开发过程中,Stuxnet微调口剂量
1Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China.
表观遗传调节器Stuxnet (Stx) 通过对抗Polycomb压制复合体1 (PRC1) 控制痕信号. 这项研究确定了一种直接的表观遗传机制,可以在发育过程中调节Notch受体水平.
科学领域:
- 发育生物学是发展生物学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子信号传递是分子信号传递.
背景情况:
- 痕信号对于发育至关重要,对受体水平敏感.
- 表观遗传调节会影响细胞对压力的反应.
- 诺奇受体剂量的直接表观遗传控制在很大程度上是未知的.
研究的目的:
- 调查表观遗传调节剂Stuxnet (Stx) 在虫发育中的作用.
- 为了确定Notch受体水平是否受到表观遗传调节.
- 为了确定控制Notch信号剂量的表观遗传机制.
主要方法:
- 研究了Stuxnet在Drosophila发育信号传递中的作用.
- 使用DamID-seq和ChIP-seq来识别Polycomb组 (PcG) 的结合部位.
- 在验证的Polycomb响应元素 (PRE) 的现场删除.
主要成果:
- 斯塔克斯网通过抵消Polycomb抑制复合体1 (PRC1) 来促进诺奇受体mRNA的表达.
- 诺奇是一个直接的PRC1目标,具有经过验证的Polycomb响应元素 (PRE).
- 删除PRE会增加诺奇表达,并导致诺奇过度激活的表型.
结论:
- 由PRC1直接进行表观遗传调节微调Notch活性剂量.
- 斯塔克斯网对抗PRC1以调节诺奇受体表达.
- 这些发现突显了基于OMICs的PCG结合在发育中的生理相关性.
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