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相关概念视频

Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with  distinct numbers of X chromosomes will...
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相关实验视频

Updated: Jul 28, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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在使用功能性Polycomb响应元件的开发过程中,Stuxnet微调口剂量.

Tao He1,2, Yu Fan1, Juan Du3

  • 1Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China.

Development (Cambridge, England)
|June 1, 2023
PubMed
概括

表观遗传调节器Stuxnet (Stx) 通过对抗Polycomb压制复合体1 (PRC1) 控制痕信号. 这项研究确定了一种直接的表观遗传机制,可以在发育过程中调节Notch受体水平.

关键词:
没有口,没有口.表观遗传调节 表观遗传调节在PRC1中,在此之前,先前前后.斯塔克斯网 (Stuxnet) 是一个网络.

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科学领域:

  • 发育生物学是发展生物学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子信号传递是分子信号传递.

背景情况:

  • 痕信号对于发育至关重要,对受体水平敏感.
  • 表观遗传调节会影响细胞对压力的反应.
  • 诺奇受体剂量的直接表观遗传控制在很大程度上是未知的.

研究的目的:

  • 调查表观遗传调节剂Stuxnet (Stx) 在虫发育中的作用.
  • 为了确定Notch受体水平是否受到表观遗传调节.
  • 为了确定控制Notch信号剂量的表观遗传机制.

主要方法:

  • 研究了Stuxnet在Drosophila发育信号传递中的作用.
  • 使用DamID-seq和ChIP-seq来识别Polycomb组 (PcG) 的结合部位.
  • 在验证的Polycomb响应元素 (PRE) 的现场删除.

主要成果:

  • 斯塔克斯网通过抵消Polycomb抑制复合体1 (PRC1) 来促进诺奇受体mRNA的表达.
  • 诺奇是一个直接的PRC1目标,具有经过验证的Polycomb响应元素 (PRE).
  • 删除PRE会增加诺奇表达,并导致诺奇过度激活的表型.

结论:

  • 由PRC1直接进行表观遗传调节微调Notch活性剂量.
  • 斯塔克斯网对抗PRC1以调节诺奇受体表达.
  • 这些发现突显了基于OMICs的PCG结合在发育中的生理相关性.