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酸乙烯和大米植物之间的相互作用:新的转化途径和代谢网络干扰

Huanhuan Xing^1,2, Xiaolong Yu¹, Jianteng Sun¹

¹Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000 Guangdong, China.

Environmental science & technology

|June 1, 2023

概括

甲酸以酸丁 (DnBP) 等甲酸以转化,产生新的化合物,影响植物新陈代谢和可能影响人类健康. 这些DnBP中间体由于塑化剂污染,对作物安全构成风险.

科学领域:

环境化学环境化学
植物新陈代谢植物新陈代谢
食品安全食品安全

背景情况:

甲酸乙是一种广泛存在的环境污染物.
了解它们与大米等主食作物的相互作用,对于评估人类暴露风险至关重要.
关于植物中酸盐的转化途径的知识有限.

研究的目的:

为了阐明大米植物中di-n-butyl甲酸盐 (DnBP) 的转化途径.
为了研究大米对DnBP暴露的代谢和蛋白质反应.
评估DnBP降解产品对作物安全的潜在风险.

主要方法:

使用超高性能液态染色学-四极点飞行时间质谱法 (UPLC-QTOF-MS) 进行非目标查.
蛋白质组分析以确定蛋白质表达的变化.
代谢分析分析,以描述代谢途径的改变.
分子对接以预测DnBP和蛋白质之间的相互作用机制.

主要成果:

确定了大米中DnBP的16种中间转化产品,包括新型化合物.
DnBP改变了大米蛋白的表达,影响了抗氧化剂的合成,应激反应和光合作用.
代谢分析显示了关键代谢途径的显著扰乱,包括酸盐循环和氨基酸代谢.
暴露于单一-n-丁甲酸盐 (MnBP) 显著改变了植物的代谢特征和分子功能.

结论:

在大米中,DnBP经历了复杂的代谢转化,产生潜在的有害中间体.
DnBP及其代谢物破坏了植物必不可少的代谢过程和分子功能.
DnBP的降解产物对植物代谢构成风险,引发了对作物安全和塑化剂污染的担忧.

关键词:

丁 - 丁 - 丁甲酸是什么?不同表达蛋白质的不同表达蛋白质.代谢网络是代谢网络.分子对接的分子对接.转化中间产品的转化中间产品.

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