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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow
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基于空间频域过的激光光斑对比成像.

Linjun Zhai1, Yongzhao Du1,2, Yuqing Fu2

  • 1School of Biomedical Science, Huaqiao University, Quanzhou, China.

Journal of biophotonics
|June 1, 2023
PubMed
概括

这项研究引入了一种新的空间频率过方法来分离静态和动态斑点,通过增强对比度和效率来改善血流成像.

科学领域:

  • 生物医学光学 生物医学光学
  • 图像处理 图像处理
  • 激光光斑成像技术 激光光斑成像技术

背景情况:

  • 激光光斑成像对于可视化血液流动至关重要.
  • 分离静态和动态斑点是具有挑战性的,但对于准确的流量量化至关重要.
  • 现有的方法可能会受到背景噪声和计算效率低下的影响.

研究的目的:

  • 开发一种用于有效分离静态和动态斑点的新方法.
  • 为了提高2D血流绘图的准确性和效率,使用激光光斑成像.
  • 提高图像质量指标,如对比度和信号噪声比.

主要方法:

  • 使用空间频率域过 (2D 里叶变换,低通和高通过).
  • 经过处理的原始斑点图像序列,每一.
  • 在时间域中平均过的序列,以获得静态和动态组件.
  • 计算的时间平均调制深度用于血流映射.

主要成果:

  • 成功地分离了静态和动态光斑组件.
  • 在幻影和体内实验中有效抑制背景不均.
  • 在图像对比度,对比度和噪声比率以及成像动态范围方面取得了显著的改进.
关键词:
富里叶变换是什么意思 富里叶变换波动调制的波动调制方法激光光斑对比图像的图像对比度空间频域过空间频域过

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  • 展示了拟议方法的高计算效率.

    • 结论:

    • 拟议的空间频域过方法有效地分离静态和动态斑点.
    • 该技术通过提高图像质量和减少噪音来增强二维血流成像.
    • 该方法为生物医学应用提供了计算效率高和强大的方法.