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内微生物群和宿主DNA之间的双向调解效应甲基化变化有助于胃腺癌

Kaile Yue^1,2, Dashuang Sheng^1,2, Xinxin Xue^1,2

¹Microbiome-X, National Institute of Health Data Science of China, Shandong University, Jinan, China.

Microbiology spectrum

|June 1, 2023

概括

胃癌 (STAD) 涉及DNA甲基化和超出Helicobacter pylori的肠道微生物. 这项研究揭示了瘤微生物组和宿主表观遗传学之间的双向联系,影响转移和预后.

科学领域:

在瘤学瘤学.
微生物学微生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

由Helicobacter pylori诱导的异常DNA甲基化是胃腺癌 (STAD) 的关键驱动因素.
非H. pylori胃微生物群在STAD病原发生中的作用仍然未被充分研究.
了解瘤微生物组与宿主表观遗传学之间的相互作用对于STAD研究至关重要.

研究的目的:

研究胃微生物组与STAD发生,转移和预后的关联.
分析与STAD转移和生存相关的差异性DNA甲基化模式.
阐明内微生物组和宿主DNA甲基化对STAD进展的双向调解效应.

主要方法:

利用了癌症基因组图谱 (TCGA) STAD项目数据,包括RNA测序,临床和DNA甲基化信息.
采用Kraken 2管道进行微生物组分析和调解分析,以评估微生物和表观遗传相互作用.
使用实时定量PCR (RT-qPCR),CCK8和细胞克隆形成试验验验证了基因表达和细胞功能.

主要成果:

确定了H. pylori和其他微生物与STAD发生,进展和预后之间的关联.
在与癌症相关的途径中发现甲基化基因的丰富,其中Kytococcus sedentarius和Actinomyces oris通过免疫基因甲基化与预后有关.
通过调节ZNF215基因表达,证明Staphylococcus saccharolyticus通过调节ZNF215基因表达促进胃细胞的增殖和克隆.

结论:

瘤微生物群-宿主表观遗传轴在STAD远端转移和患者存活中发挥着关键作用.
内微生物及其代谢物可以影响宿主DNA甲基化,提供潜在的治疗点.
描述微生物物种和功能基因对于了解它们对胃癌发展的贡献至关重要.

关键词:

通过DNA甲基化.胃肠道的微生物群主体-微生物相互作用胃癌胃癌是一种癌症.瘤微环境是一个微环境.

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