作为Entamoeba histolytica中可能的乙胆受体的Gal/GalNac 莱克
Marisol Pacheco-Sánchez1, Sandra Luz Martínez-Hernández2, Martín Humberto Muñoz-Ortega3
1Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Mexico.
Frontiers in cellular and infection microbiology
|June 1, 2023
概括
Entamoeba histolytica使用其 Gal/GalNAc 莱克结合乙胆 (ACh),激活信号通路. 这种相互作用增强了寄生虫的毒性,并有助于侵入性虫病的发展.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 每年有数以百万计的Entamoeba histolytica会导致阿米比亚सिस,入侵是由宿主免疫分子相互作用促进的.
- 建议乙胆 (ACh) 与E. histolytica膜的相互作用增加毒性因子.
研究的目的:
- 为了识别E. histolytica中特定的膜受体,负责结合ACh. trophozoites的特异性受体.
- 为了阐明由 ACh 结合激活的下游信号通路.
主要方法:
- 免疫光剂用于 ACh 和 Gal/GalNAc 莱克的同位化.
- 西方污染和生物信息学分析.
- 通过RT-qPCR量化Ras和Rab GTPase的表达.
主要成果:
- /GalNAc 列蛋白,可能通过其 150 kDa 的子单元,作为 ACh.的结合部位.
- ACh结合激活了Ras和Rab GTPases,这些酶对菌的增殖,细胞骨和囊泡贩运至关重要.
- 这种相互作用促进了E. histolytica的致病性.
结论:
- 组织分析菌通过其Gal/GalNAc lectin积极捕获ACh.
- ACh结合会触发信号级联,这对于寄生虫的毒性和侵入性虫病至关重要.
- 针对这种相互作用可以提供新的治疗策略.
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