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在空间异质的生物膜环境中,纳米粒子的异常扩散

Bart Coppens¹, Tom E R Belpaire¹, Jiří Pešek²

¹Division of Mechatronics, Biostatistics, and Sensors, KU Leuven, 3001 Leuven, Belgium.

|June 1, 2023

概括

功能化纳米粒子 (NP) 显示出由于结构和细胞外聚合物质 (EPS) 变化的细菌生物膜中的多种扩散. 了解这些因素可以优化NP的抗菌药物输送策略.

科学领域:

微生物学微生物学
材料科学材料科学材料科学
生物物理学的生物物理.

背景情况:

包裹在细胞外聚合物质 (EPS) 中的细菌生物膜,由于透率有限,对抗微生物治疗提出了重大挑战.
功能化纳米粒子 (NP) 被探索为有针对性的抗微生物药物输送的先进载体,以克服生物膜障碍.

研究的目的:

为了研究纳米颗粒在沙门氏菌生物膜中的扩散动态.
为了将纳米粒子分布与生物膜结构和细胞外聚合物质 (EPS) 含量相关联.
评估纳米粒子扩散对抗微生物药物输送的影响.

主要方法:

使用显微镜观察和分析沙门氏菌生物膜中的纳米粒子扩散.
布朗的动力学建模,结合了细菌周围的空间变化的粘度,被用来模拟纳米粒子的行为.
该模型与观察到的纳米粒子分布和扩散模式进行了验证.

主要成果:

生物膜内的纳米粒子扩散表现出异常的行为和异质的移动性,受生物膜结构的影响.
观察到不同的纳米粒子分布,与生物膜架构的变化直接相关.
布朗的动力学建模成功地复制了观察到的扩散特征和垂直纳米粒子分布,确定了细菌附近的粘性水槽.

结论:

生物膜结构和细胞外聚合物物质 (EPS) 水平极大地影响了纳米粒子药物递送效率.
较低的EPS水平可以通过将纳米颗粒定位在细菌附近来增强抗菌剂的传递.
由于屏蔽效应,更高的EPS度可能会阻碍纳米颗粒的传递,需要量身定制的策略.

关键词:

纳米颗粒纳米颗粒纳米科学是一个纳米科学.纳米技术纳米技术

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