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活体共聚焦显微镜检测急性和慢性病变的基本模式,使用新鲜的脏样本
Jesús Z Villarreal1,2, Javiera Pérez-Anker2,3, Susana Puig3
1Department of Nephrology and Renal Transplantation, Hospital Clínic de Barcelona, Institut d'Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, Barcelona, Spain.
Clinical kidney journal
|June 1, 2023
概括
活体共聚焦显微镜 (FuCM) 为病理学提供实时,高分辨率的成像. 这种技术与评估病变的传统方法有很强的一致性,有可能改善诊断工作流程.
科学领域:
- 腎病理學 腎病理學
- 医疗成像医学成像
- 组织病理学 组织病理学
背景情况:
- 活体共聚焦显微镜可以实时提供新鲜组织的高分辨率成像.
- 它的光学分辨率可以与传统的组织病理学相比较.
- 这项研究探讨了聚变模式共聚焦显微镜 (FuCM) 和其类似于H&E的数字染色用于病理学.
研究的目的:
- 调查使用FuCM用于病理学的可行性.
- 为了评估类似于H&E的数字染色,用于分析脏组织中的基本病变模式.
- 为了比较FuCM的发现与传统的组织病理学.
主要方法:
- 使用第四代ex vivo共聚焦显微镜装置分析了48个脏样本.
- 样品接受了FuCM成像和常规处理.
- 使用百分比协议和kappa指数来评估一致性.
主要成果:
- 在H&E类数字染色和常规显微镜之间,对于急性管状损伤,发现了强烈的一致性 (κ=0.88).
- 观察到间歇性纤维化,炎症和血管病变的实质性一致性 (κ=0.79).
- 此外,FuCM还可以快速识别额外毛囊增殖,亡和细分性硬化症.
结论:
- 在评估新鲜脏组织中的急性管状缩和间歇性纤维化方面,FuCM与传统技术一样有效.
- 图像获取的方便性表明FuCM对于快速脏活检评估的实用性.
- 富CM有潜力优化组织病理学的工作流程.
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