超疏水性旋转芯片用于对耐药细菌的计算机视觉识别
Jiacheng He1, Ruonan Peng2, Henry Yuqing3
1Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, New York 14623, United States.
ACS applied materials & interfaces
|June 1, 2023
概括
这项研究介绍了旋转芯片,这是一种用于检测病原体的新型微流体装置. 它可以在没有外部仪器的情况下实现多重反应和准确的结果,非常适合 point-of-care 设置.
科学领域:
- 微流体学 微流体学
- 临床诊断 临床诊断 临床诊断 临床诊断
- 病原体检测检测 病原体检测
背景情况:
- 微流体系统通常需要外部和门,限制其在护理点 (POC) 设置中的使用.
- 高效的试剂运输和混合对于微流体分析至关重要.
研究的目的:
- 开发一种简单的,无需仪器的微流体系统,用于多重病原体检测.
- 为了使试剂转移和反应执行使用手动旋转用于POC应用.
主要方法:
- 使用计算机数控 (CNC) 机械加工制造旋转芯片.
- 利用同心层的手动旋转来进行试剂转移.
- 超性涂层的应用,以实现高效的流体输送.
- 与细胞内光试验的整合用于病原体检测.
- 开发用于自动化结果分析的计算机视觉方法.
主要成果:
- 实现了92.78%的流体运输效率,具有超疏水涂层,没有可观察到的泄漏.
- 确立了1.8 × 10^6 CFU/mL的芯片上检测极限,用于对安培素耐药的*Escherichia coli* (*E. coli*).
- 在使用计算机视觉方法区分阳性和阴性样本时,证明了100%的准确性.
结论:
- 旋转芯片是一种简单,低成本,高通量微流体装置.
- 它促进了多重反应和自动结果显示,适用于资源有限的POC应用程序.
- 无仪器设计使其非常适合现场部署和各种诊断需求.
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