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与变相关的转录的特定处理与人类卵细胞的最终成熟有关

Sara Pietroforte^1,2, Montserrat Barragan Monasterio¹, Anna Ferrer-Vaquer^1,3

¹Basic Research Laboratory - Eugin Group, Barcelona, Spain.

Molecular human reproduction

|June 1, 2023

概括

人类卵细胞成熟涉及动态RNA处理,特别是3'未翻译区域 (UTR) 缩短. 这种转录重塑对于调节基因翻译和实现成功的介质进展至关重要.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

人类卵细胞成熟需要精确的转录组调节才能获得繁殖成功.
晚期半变异期间的同转录和后转录mRNA处理仍然不太了解.
这些过程会影响转录组复杂性和翻译速率.

研究的目的:

为了研究在人类卵细胞变质过程中的mRNA处理和基因表达变化.
为了确定关键的RNA处理事件,对中性成熟至关重要.
了解转录重塑在卵细胞发育中的作用.

主要方法:

在不同介质阶段 (体内和体外成熟) 的40个人类卵细胞的RNA测序.
对基因表达,拼接和mRNA前处理的分析.
通过不同成熟时间对RNA处理进行比较.

主要成果:

显著的3个未翻译区域 (UTR) 处理,主要是3个UTR缩短,观察到从生殖囊泡 (GV) 到第II (MII) 阶段的与微变相关的基因.
结合体和前mRNA处理基因的差异表达支持观察到的RNA修饰.
在MII之前GV卵细胞的最小差异表明RNA处理与成功的介质过渡有关.
染色体组织和螺旋组装基因的蛋白质异型中微小但特定的变化.

结论:

人类雌性半变异包括动态转录重塑.
预mRNA处理,特别是3 UTR缩短,驱动选择性翻译调节.
这些RNA的修改对于实现最终的介质成熟至关重要.

关键词:

3′未翻译的区域加工 3′未翻译的区域.替代性拼接是一种替代性的拼接.基因表达的基因表达方式人类卵子细胞的人类卵子细胞介质变化 (meiosis) 是一种变质的过程.卵子细胞的能力能力.拼接拼接拼接拼接

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