粘膜转录突出了与性结肠炎,克罗恩病和腹腔疾病有关的lncRNAs
Tzipi Braun1, Katya E Sosnovski1,2, Amnon Amir1
1Sheba Medical Center, Tel-Hashomer, affiliated with the Tel Aviv University, Tel Aviv, Israel.
JCI insight
|June 1, 2023
概括
这项研究确定了在性结肠炎 (UC) 和克罗恩病 (CD) 等炎症性肠病中失调的长非编码RNA (lncRNAs). 降低HNF1A-AS1 lncRNA水平与疾病严重程度相关,并影响肠道健康.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 性结肠炎 (UC),克罗恩病 (CD) 和腹腔炎是常见的炎症性肠道疾病,治疗选择有限.
- 长非编码RNAs (lncRNAs) 是有前途的治疗点,因为它们的细胞特异性表达和功能作用.
研究的目的:
- 在不同的炎症状况中分析肠道粘膜中的IncRNA表达.
- 确定和优先考虑与UC疾病严重程度和结果相关的lncRNAs.
- 探索lncRNAs,肠道微生物和粘膜平衡之间的联系.
主要方法:
- 从696个受试者的肠道粘膜转录组的统一分析.
- 在未接受治疗的UC,CD和乳病队伍中识别失调的lncRNAs.
- 使用PROTECT队列和与肠道微生物群的相关性来优先考虑与UC相关的lncRNAs.
主要成果:
- 与蛋白质编码基因相比,lncRNAs在肠道中表现出更多的位置特异性表达.
- 在UC,CD和腹腔疾病中发现了失调的IncRNAs.
- 在所有三种条件下,HNF1A-AS1 lncRNA都减少了,严重的UC进一步减少;在小鼠中,它的耗尽增加了结肠炎的易感性.
结论:
- 失调的lncRNAs,特别是HNF1A-AS1,代表了肠道炎症疾病的潜在治疗点.
- lncRNA概况可以告知UC的疾病严重程度和结果.
- 需要进一步的临床前研究来研究 lncRNAs 作为治疗点.
相关概念视频
lncRNA - Long Non-coding RNAs
8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
Inflammatory Bowel Disease I: Ulcerative Colitis
227
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
227
Inflammatory Bowel Disease II: Crohn's Disease
306
Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
306
Mucosal Barrier of the Stomach
730
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
730
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
365
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
365
Renewal of Intestinal Stem Cells
2.6K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.6K


