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Updated: Jul 28, 2025

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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通过PLOD1 lysine hydroxylase调节EBNA1蛋白稳定性和DNA复制活性

Jayaraju Dheekollu1, Andreas Wiedmer1, Samantha S Soldan1

  • 1The Wistar Institute, Philadelphia, Pennsylvania, United States of America.

PLoS pathogens
|June 1, 2023
PubMed
概括

聚合物氨酸二氧化酶1 (PLOD1) 调节爱斯坦-巴尔核抗原1 (EBNA1) 的稳定性和功能. 对于EBNA1在病毒复制,发作维护和宿主细胞存活中的作用来说,PLOD1的相互作用至关重要.

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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
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