通过将路径模块化和运输工程相结合,高效地进行d-Tocotrienol的分泌生产
Xue Jiao1, Qi Bian1,2, Taotao Feng1
1Key Laboratory of Biomass Chemical Engineering (Education Ministry), College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310058, China.
Journal of agricultural and food chemistry
|June 1, 2023
概括
这项研究设计了一个酵母细胞工厂,用于高效的δ-托克二醇生产. 优化的微生物过程实现了这种有价值的维生素E化合物的高产量和分泌.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物生产 微生物生产
背景情况:
- 德尔塔-托科特里诺 (δ-TCT) 具有显著的辐射保护,神经保护和降胆固醇的特性.
- 自然 δ-TCT 生产受到植物含量低和复杂的提取工艺的限制.
- 微生物发酵为生产 δ-TCT.提供了一个可持续的替代方案.
研究的目的:
- 开发一个微生物细胞工厂,专门生产和分泌 δ-TCT.
- 为了改造Saccharomyces cerevisiae以提高δ-TCT生物合成和恢复.
- 优化发酵条件以最大限度地提高细胞外 δ-TCT 产量.
主要方法:
- 在Saccharomyces cerevisiae中组装了δ-TCT合成途径.
- 进行了全面的代谢工程和途径优化.
- 使用了2-基-β-环氧 (CD) 和橄油,用于增强生产和提取.
主要成果:
- 通过路径工程实现了73.45 mg/L的初始 δ-TCT标位.
- 升高的δ-TCT标位达到241.7 mg/L (63.65 mg/g干细胞重量),CD和PDR1过度表达.
- 使用CD和in situ橄油提取获得181.12 mg/L的细胞外 δ-TCT,占总产量的85.6%.
结论:
- 建立了一个强大的酵母细胞工厂,用于高产量 δ-TCT 生产.
- 证明了成功的工程对于 δ-TCT 的分泌生产.
- 为酵母工程提供了洞察力,以实现可扩展的 terpenoid 生产.
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