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在过敏性鼻炎中,MiR-29a-3p通过直接准CTNNB1-VCL模块来促进鼻上皮质屏障功能障碍

Na Wang¹, Pu Li¹, Junqi Liu¹

¹Department of Otorhinolaryngology Head and Neck Surgery, XuanWu Hospital, Capital Medical University, No. 45, Changchun Street, Beijing 100053, PR China.

International immunopharmacology

|June 1, 2023

概括

微RNA-29a-3p (miR-29a-3p) 在过敏性鼻炎 (AR) 中被上调,准VCL和CTNNB1,以破坏鼻上皮质屏障功能. 抑制miR-29a-3p可能为AR提供新的治疗策略.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

过敏性鼻炎 (AR) 涉及IgE介导的反应,导致鼻腔粘膜炎症和表皮屏障完整性受损.
miR-29a-3p在AR病变发生中的作用及其对表皮屏障功能的影响仍然不清楚.

研究的目的:

为了研究miR-29a-3p在过敏性鼻炎的发病过程中的作用.
为了确定参与调节鼻上皮质屏障功能的miR-29a-3p的目标基因.

主要方法:

在AR患者和OVA诱导的AR小鼠模型中对miR-29a-3p表达的定量分析.
生物信息预测和双露西法酶记者测定用于验证miR-29a-3p目标基因 (VCL和CTNNB1) 的有效性.
使用人类鼻上皮细胞 (HNEpC) 的体外实验和在小鼠中使用miR-29a-3p对抗胺的体内研究.

主要成果:

在AR患者和鼻上皮细胞中,miR-29a-3p显著上调.
VCL和CTNNB1被确定为miR-29a-3p的直接目标,对于粘附和紧密结合至关重要.
在体外和体内抑制miR-29a-3p部分恢复了鼻上皮质屏障功能,并减轻了小鼠的AR症状.

结论:

miR-29a-3p针对CTNNB1和VCL,以调节鼻上皮质透性和屏障完整性.
miR-29a-3p代表了对过敏性鼻炎治疗的潜在新型治疗标.

关键词:

过敏性鼻炎是一种过敏性鼻炎.在 CTNNB1 找皮质屏障是一种表皮屏障.在 VCL VCL 中.在 miR-29a-3p 中.

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