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Updated: Jul 28, 2025

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

在微通道中使用三元粒子分离的数值调查,在微通道中使用壁上安装的障碍物,使用介电泳

Reza Derakhshan¹, Ali Bozorgzadeh¹, Abas Ramiar¹

¹Mechanical Engineering Department, Microfluidics and MEMS lab, Babol Noshirvani University of Technology, Babol, Mazandaran, Iran.

Journal of chromatography. A

|June 1, 2023

概括

这项研究介绍了一种无盖的3D微流体装置,使用压电泳 (DEP) 精确分离三种不同的聚乙烯颗粒大小. 优化的设计和操作参数使得实验室在芯片上的应用程序能够高效地进行三元粒子分离.

科学领域:

微流体学和纳米技术
生物物理学和生物医学工程

背景情况:

微流体粒子操纵对于科学进步至关重要.
压电泳 (DEP) 提供了一种高效,精确和无标签的粒子操纵方法.

研究的目的:

使用DEP设计和研究一个带有形障碍的无盖3D微通道,用于使用DEP连续流分离三个聚乙烯颗粒群 (5,10和20微米).
开发和验证一个OpenFOAM代码,用于模拟微通道内的粒子运动.
以数值研究操作和几何参数对颗粒分离效率的影响.

主要方法:

开发一个无盖的3D微通道,配有挂在墙上的梯形障碍物.
使用介电泳 (DEP) 进行粒子操纵和分离.
使用OpenFOAM进行数值模拟和粒子运动的实验验证.
对障碍物高度,应用于电压,电极角度和流速的参数研究.

主要成果:

开发的OpenFOAM解决器表现出高准确度,经过实验测试验证.
有效的颗粒分离需要障碍物高度超过350微米.
分离的最佳电压范围受到电极角度和流速的影响.
该系统可以在10V处理2.5μL/分钟,实现同时三元分离.

结论:

拟议的微流体设计允许精确,无标签,连续流的分离多个粒子群体.
无盖,简单的设计适合集成到芯片上的实验室设备和临床诊断.
优化操作和几何参数是实现高分离效率的关键.

关键词:

电介电泳是电介电泳.芯片上的实验室,芯片上的实验室微流体学微流体学基于尺寸的分离方式三级粒子分离技术

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