肠道微生物组的组成与克罗恩氏病在健康的一级亲属的未来发病有关
Juan Antonio Raygoza Garay1, Williams Turpin2, Sun-Ho Lee1
1Division of Gastroenterology & Hepatology, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Zane Cohen Center for Digestive Diseases, Mount Sinai Hospital, Toronto, Ontario, Canada.
Gastroenterology
|June 1, 2023
概括
这项研究确定了一种肠道微生物群的特征,可以预测健康亲属未来的克罗恩病 (CD) 发病. 这一发现表明肠道微生物组在CD发育中起作用.
科学领域:
- 胃肠病学 胃肠病学
- 微生物组研究 微生物组研究
- 计算生物学 计算生物学
背景情况:
- 克罗恩病 (CD) 的病因仍然未知,目前的假设涉及微生物/环境因素和遗传易感性之间的相互作用.
- 之前的病例控制研究在CD患者中发现了肠道微生物组合的改变,但无法确定因果关系或时间关系.
- 区分在CD发作之前的微生物组变化与因炎症或治疗而导致的微生物组变化至关重要.
研究的目的:
- 确定特定的肠道微生物组合物,这些成分在克罗恩病 (CD) 发病之前就存在.
- 确定肠道微生物组组成对CD发展风险的预测值.
- 建立与未来CD开发相关的微生物签名.
主要方法:
- 一项前性队列研究,涉及3483名CD患者的健康一级亲属.
- 应用机器学习方法来分析肠道微生物组合,使用16S核糖体RNA测序.
- 在一个独立的队列中验证开发的微生物组风险评分 (MRS) 模型.
主要成果:
- 微生物组风险评分 (MRS) 模型证明了预测价值,在验证队列中,危险率为2.24 (P = .04).
- MRS显示了时间有效性,识别了在诊断前5年内患有CD的人 (AUC > 0.65).
- 导致MRS的关键分类包括Ruminococcus torques,Blautia,Colidextribacter,一个未经培养的奥斯基洛斯皮拉属,以及Roseburia.
结论:
- 这项研究提供了第一个证据,将肠道微生物组组成与克罗恩病的未来发病联系起来.
- 这些发现表明,肠道微生物组是CD病变的致病因素.
- 开发的MRS可能有助于早期识别CD的风险.
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