在细分的核糖体内RNA/RNA相互作用区域的祖先
1Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001, USA.
概括
在原生体中,细分的核糖体利用RNA/RNA相互作用来保持稳定性,这表明古老的起源早于DNA. 这些相互作用对于核糖体组装和在各种生命形式中的功能至关重要.
科学领域:
- 分子生物学分子生物学
- 进化生物学 进化生物学
- 生物化学 生物化学
背景情况:
- 核糖体,蛋白质合成机器,是普遍保留的核糖体.
- 大亚单元核糖体RNA的细分在像类核糖体动物这样的原生体中被观察到,这对核糖体结构和功能构成挑战.
- 以前,人们认为RNA/蛋白相互作用是唯一负责稳定碎片化的核糖体的.
研究的目的:
- 为了研究来自试虫和Euglena gracilis的细分核糖体中的保存的RNA/RNA相互作用区域.
- 通过与未细分的核糖体进行比较,确定这些RNA/RNA相互作用的进化年龄.
- 探索RNA的2'OH组在稳定这些相互作用中的作用.
主要方法:
- 对细分核糖体的冷EM结构进行比较分析.
- 分析来自真核生物,细菌和古生物的非细分核糖体的晶体结构.
- 检查结模式,特别是涉及2'OH组.
主要成果:
- 在tripanosomatid和Euglena gracilis细分的核糖体中证实了保留的RNA/RNA相互作用区域,尽管细分模式不同.
- 分析表明,这些保存的RNA/RNA相互作用是古老的,可能起源于所有生命的最后一个共同祖先.
- 发现RNA的2'OH组主要负责促进这些相互作用中的单一键.
结论:
- RNA/RNA相互作用在细分核糖体的折叠和稳定中发挥着重要作用,甚至在蛋白质结合之前.
- 这些RNA/RNA相互作用的古老性质为早期核糖体进化提供了洞察力.
- 2'OH介导的键的流行支持了RNA早于DNA的假设.
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