洞察冷血与联合转录组学和代谢组学治疗的潜致死性Listeria monocytogenes的无活化和反应机制
Yuan-Yuan Pan1,2,3,4, Da-Wen Sun1,2,3,5, Jun-Hu Cheng1,2,3
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Journal of applied microbiology
|June 1, 2023
概括
冷血会在Listeria monocytogenes中引发氧化应激,改变代谢途径以使其无活化并降低病原性. 这项多学科研究揭示了微生物控制的关键机制.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 等离子体物理学的物理学
背景情况:
- 李斯特菌 (Listeria monocytogenes) 构成一个重要的食品安全风险.
- 冷等离子体为微生物无活化提供了一种非热方法.
- 了解冷等离子体诱导失活化的分子机制对于有效应用至关重要.
研究的目的:
- 为了研究Listeria monocytogenes对冷血的分子反应.
- 为了阐明在冷等离子体诱导的氧化应激下无活化机制.
- 通过多omics方法分析细菌适应.
主要方法:
- 高通量转录学和代谢学分析.
- 对基因和代谢物表达模式的研究.
- 对细胞通路和抗氧化剂系统的分析.
主要成果:
- 冷血诱导了跨膜运输,铁流量和氨基酸代谢的显著变化.
- 细菌铁和病原性衰减是由细胞内氧化应激介导的.
- 观察到典型的抗氧化系统 (例如,TrxR-Trx) 的抑制和像谷氨代谢这样的替代途径的激活.
结论:
- 冷血诱导的细胞内氧化应激扰乱了关键通路,导致Listeria monocytogenes失活并降低了病原性.
- 该研究提供了多维的洞察力,用于等离子体非热处理的微生物失活机制.
相关概念视频
01:23Physical Methods for Controlling Microbial Growth: Temperature
151
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
151
01:23Stringent Response in E. coli
32
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
32
01:28Biological Methods for Microbial Control
145
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
145
01:30Other Stress Responses in Bacteria
34
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
34