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检测释放CO的分子 (CORM) 不等于检测CO:DPHP和CORM-3的情况

Dongning Liu¹, Xiaoxiao Yang¹, Binghe Wang¹

¹Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, Georgia 30303, United States.

Analytical chemistry

|June 1, 2023

概括

作为一氧化碳 (CO) 探针的DPHP-Cu(II) 系统无法检测二氧化碳气体,并与其他化学物质发生反应. 这项研究强调了在开发新的CO探测器时需要可靠的CO来源和验证.

科学领域:

生物化学和化学生物学
分析化学分析化学
分子探测器和传感器

背景情况:

一氧化碳 (CO) 是一种具有显著药理作用的内源信号分子.
开发灵敏和选择性的光探针对于研究CO生物学至关重要.
通常使用的CO释放分子 (CORM) 可能具有不可靠的CO释放和固有的反应性,可能导致探针开发中的错误阳性.

研究的目的:

批判性地评估DPHP-Cu(II) 系统作为一氧化碳 (CO) 的光探针.
确定CORM-3报告的光恢复是否是由于CO检测或其他化学反应.
评估DPHP-Cu系统对二氧化碳对硫化物等其他物种的选择性.

主要方法:

使用纯一氧化碳 (CO) 气体和CORM-3作为CO来源进行实验验证.
光光谱法用于监测DPHP-Cu (II) 系统的响应.
测试系统的反应性,使用降解剂,如酸盐和硫化物种.

主要成果:

DPHP-Cu(II) 系统对CORM-3显示光反应,与之前的报道一致.
然而,该系统对纯一氧化碳 (CO) 气体没有反应.
光激活也被观察到与酸盐,表明缺乏CO选择性,并建议反应是由于减少Cu (II) 而不是CO检测.

结论:

该DPHP-Cu (II) 系统不是选择性CO探头,不能可靠地用于研究CO生物学.
观察到的光变化很可能是由于CORM-3和其他降解剂的固有反应性,而不是CO.
未来开发的CO探头必须使用纯的CO气或验证的CO来源,并进行严格的测试,以确保真正的CO选择性并避免人工物.

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