里奥1降低了中心基RNA水平,以促进结构适合的基因合体的及时组装
Ksenia Smurova1, Michela Damizia1, Carmela Irene1
1Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, 38123, Trento, Italy.
Nature communications
|June 1, 2023
概括
蛋白质激酶Rio1调节酵母中的中粒体转录 (cenRNAs) 和周边中心体非编码RNA (percenRNAs). 里约热内卢1 里约热内卢1
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 运动细胞组件的组装依赖于CENP-A和中间体转录 (cenRNAs).
- 通过降低RNA聚合酶II (RNAPII) 和核外体介导的周转率来维持低cENRNA水平.
- 在这个过程中,特定蛋白激酶的作用在很大程度上仍未被探索.
研究的目的:
- 研究蛋白激酶Rio1在Saccharomyces cerevisiae中调节cenRNAs和pericenRNAs中的功能.
- 确定Rio1对中粒体和动粒体形成以及染色体稳定性的影响.
主要方法:
- 使用Saccharomyces cerevisiae作为一个模型生物体.
- 研究Rio1耗尽对cenRNA和pericenRNA水平的影响.
- 在野生类型和Rio1贫乏酵母中评估了动态基因组组,CEN核细胞形成和染色体稳定性.
- 结果与缺乏Rio1正义细胞RioK1.1的人类细胞进行了比较.
主要成果:
- 酵母在1:1的比例下产生短和长的cenRNAs.
- 里约1通过降低RNAPII可访问性来限制CENRNA的产生,并通过Rat1促进降解.
- 此外,Rio1还抑制了非编码的危险RNAs,这些非编码的危险RNAs比 cenRNAs 更丰富.
- 减少Rio1导致CEN和PERICENRNA的积累,CEN核细胞和基因组的错误形成,以及染色体的不稳定.
- 在缺乏RioK1.1的人类细胞中观察到类似的表型.
结论:
- 蛋白激酶Rio1在控制酵母中中心基和周心基非编码RNA水平方面发挥着至关重要的作用.
- 里奥1对于适当的动态细胞组合和染色体稳定性至关重要.
- 涉及Rio1/RioK1参与中心体调节的调节机制在进化上是保留的.
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