刺夜的染色体层次基因组组合和注释Solanum rostratum Dunal Dunal的刺夜
Yue Zhang1, Wenchao Guo2, Zhili Yuan1
1Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Scientific data
|June 1, 2023
概括
创建了刺夜 (Solanum rostratum) 的染色体级基因组组. 这种基因组资源有助于研究基因组的适应性和对农业的影响.
科学领域:
- 基因组学就是基因组学.
- 植物生物学 植物生物学
- 侵入性物种研究 侵入性物种研究
背景情况:
- 刺夜 (Solanum rostratum) 是一种来自北美的破坏性入侵性杂草,现在在34个国家发现.
- 它对全球生态系统,农业,畜牧业和人类健康构成重大威胁.
研究的目的:
- 构建一个高质量的,染色体水平的基因组组装和Solanum rostratum的注释.
- 为了解其生物学和生态影响提供基础的基因组资源.
主要方法:
- 利用PacBio的高保真度读取用于初始基因组装.
- 采用了Hi-C测序数据,用于对12个伪染色体的支架和定序列.
- 进行基因预测和注释,包括识别非编码RNA和重复序列.
主要成果:
- 产生了一个869.69 Mb的基因组组合,定在12个伪染色体上,具有72.15 Mb的N50结合.
- 确定了649.92 Mb的重复序列和3588个非编码RNA.
- 预测了29694个编码蛋白质的基因,其中94.81%的功能注释和高完整性胚胎细胞_odb10基因 (99.5%).
结论:
- 索拉 (Solanum rostratum) 的染色体级基因组组合是一个重要的基因组资源.
- 这种资源将促进未来研究杂草的环境适应机制.
- 它还将有助于研究与科罗拉多马甲虫等害虫相关的宿主转移.
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