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来自肠道微生物群的脂质代谢物促进调控性T细胞分化

Hiroaki Shiratori¹, Hiroyuki Oguchi^1,2, Yosuke Isobe^2,3

¹Division of Biochemistry, Department of Pharmaceutical Sciences, Faculty of Pharmacy, and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo, 105-8512, Japan.

Scientific reports

|June 1, 2023

概括

肠道细菌产生脂质,有助于免疫细胞的发展. 研究人员确定了两个特定的脂质,9,10-DiHOME和全转网红酸,它们诱导调节性T细胞,这对免疫平衡至关重要.

科学领域:

免疫学免疫学免疫学
微生物组研究的研究.
代谢学代谢学代谢学

背景情况:

共生细菌的代谢物极大地调节宿主免疫力.
虽然水友性代谢物的作用已知,但肠道微生物群衍生脂质的免疫调节作用需要进一步研究.

研究的目的:

为了研究肠道微生物群衍生脂质的免疫调节作用.
为了确定负责诱导调节性T细胞 (Tregs) 的特定脂质.

主要方法:

从无特定病原体 (SPF) 和无细菌 (GF) 的小鼠便中提取脂质.
使用逆相高性能液态染色学 (RP-HPLC) 进行分离.
通过液体染色学-并联质谱法 (LC-MS/MS) 进行脂质组概况分析.

主要成果:

来自SPF小鼠的便脂质提取物,但不是GF小鼠,表明Treg诱导活性.
两种生物活性脂质,9,10-dihydroxy-12Z-octadecenoic acid (9,10-DiHOME) 和全转录氨酸 (atRA),被确定具有体外Treg诱导能力.
在硫酸 (DSS) 诱导的大肠炎模型中,光线9,10-DiHOME水平显著下降.

结论:

结合性细菌衍生的脂友性代谢物,包括9,10-DiHOME和atRA,可以促进Treg的发育.
9,10-DiHOME可能作为大肠炎的潜在生物标志物.

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