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早期miR-320b和miR-25-3p小RNA水平与10年多发性硬化症的严重程度相关:一个队列研究

Alicia Gonzalez-Martinez¹, Gauruv Bose^1,2,3, Hrishikesh Lokhande¹

¹Translational Neuroimmunology Research Center (TNRC), Ann Romney Center for Neurologic Diseases (ARCND), Department of Neurology, Brigham and Women's Hospital, 60 Fenwood Road, 9002K, Boston, MA, 02115, USA.

Journal of neuroinflammation

|June 1, 2023

概括

早期检测多发性硬化症 (MS) 障碍是可以使用特定的微RNAs (miRNAs). 血清中的miR-320b和miR-25-3p水平可以预测长期的MS严重程度和大脑缩.

科学领域:

神经科学是一个神经科学.
生物标志物生物标志物
免疫学免疫学免疫学

背景情况:

多发性硬化症 (MS) 是一种慢性自身免疫性疾病,导致逐渐残疾.
微RNAs (miRNAs) 正在成为MS的潜在生物标志物.
很少有研究将早期miRNA表达与长期MS结果联系起来.

研究的目的:

研究血清miR-320b,miR-25-3p和miR-486-5p作为MS残疾的早期生物标志物.
评估这些miRNA与长期多发性硬化症结局在10年随访期间之间的关联.

主要方法:

分析了发病后三年内144名多发性硬化症患者的血清样本.
使用RT-PCR量化了miRNA的表达.
在10年内与良性多发性硬化症 (EDSS ≤2) 的关联是主要结局.

主要成果:

较低的miR-25-3p和较高的miR-320b水平与保持良性MS相关.
较高的miR-320b水平与发展次级渐进性MS (SPMS) 相关联.
10岁时大脑辅酶分数与miR-25-3p负相关,与miR-320b正相关.

结论:

血清miR-320b和miR-25-3p是与MS严重程度和大脑缩相关的早期生物标志物.
这些miRNA可能有助于风险分层的MS患者及时做出治疗决策.
miR-486-5p和T2损伤体积与长期结果无关.

关键词:

生物标志物生物标志物障碍障碍是指残疾人的残疾.在EDSS中使用EDSS.长期的长期的长期的多发性硬化症是多发性硬化症.在RRMS中使用RRMS.这就是SPMS的原因.在 miR-25-3p 中使用.在 miR-320bb 中使用.这是一个小RNARNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...