将病原体添加到复杂的口腔微生物群中,导致生物膜组成和功能发生显著变化
Yuan Liu1, Scott G Daniel2, Hye-Eun Kim1
1Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Microbiome
|June 1, 2023
概括
严重的幼儿 (S-ECC) 涉及突变菌和白菌. 这项研究使用了一种新的生物膜模型来展示这些病原体和糖如何改变口腔微生物群,可能导致疾病的发展.
科学领域:
- 口腔微生物学 口腔微生物学
- 牙科研究 牙科研究
- 生物膜的动力学
背景情况:
- 牙损是一种由糖和微生物驱动的生物膜疾病.
- 严重的幼儿 (S-ECC) 涉及突变菌和白菌.
- 口腔斑块跨王国相互作用的机制尚不清楚.
研究的目的:
- 研究外源性病原体如何影响口腔微生物群.
- 评估本地口腔微生物群的结构和功能变化.
- 使用一种新的ex vivo唾液衍生微观宇宙生物膜模型.
主要方法:
- 唾液衍生的生物膜的整个元基因组测序.
- 添加了Streptococcus mutans和/或Candida albicans. 这些细菌.
- 在不同的糖条件下分析微生物组的组成,多样性和糖代谢.
- 同焦显微镜成像.
主要成果:
- 与浮游生物相相比,生物膜显示丰富度/多样性减少,糖代谢增加.
- 外源病原体显著改变了细菌的组成,其影响因糖类而异.
- 在糖糖下S. mutans和C. albicans的组合诱导了微生物群和KEGG通路的独特变化.
结论:
- 单独或与糖相结合的S. mutans和C. albicans能够独特地改变生物膜的组成和功能.
- 这种S. mutans-C. albicans组合可能会驱动失生性和空性口腔微生物组.
- 该模型有助于研究功能性微生物组和开发调制策略.
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