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全基因组关联研究揭示了在高光适应过程中,PAS/LOV蛋白对酸盐的调节

Fayezeh Aarabi¹, Andrea Ghigi¹, Micha Wijesingha Ahchige¹

¹Central Metabolism, Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm 14476, Germany.

Plant physiology

|June 2, 2023

概括

在阿拉比多普西斯的高光适应过程中,PAS/LOV蛋白 (PLP) 通过与维生素C缺陷2 (VTC2) 相互作用来调节酸盐水平. 蓝光减弱了这种相互作用,影响了压力期间的甲酸盐积累.

科学领域:

植物生理学植物生理学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

光线条件显著影响植物的代谢反应,酸盐水平在适应过程中起着关键作用.
了解控制压力下的亚斯科巴酸盐平衡的遗传和分子机制对于植物适应至关重要.

研究的目的:

为了确定与高光 (HL) 适应期间与酸盐水平调节相关的遗传位点在Arabidopsis thaliana.
阐明涉及HL诱导的酸盐变化的分子相互作用和信号通路.

主要方法:

全基因组关联研究 (GWAS) 在315个Arabidopsis加入.
在强光条件下分析酸盐含量.
亚细胞局部化,酵母双杂交和双分子光补充试验.
对PAS/LOV PROTEIN (PLP) 和加密染色体的淘汰突变的表征.

主要成果:

在GWAS中,发现了显著的单核酸多态 (SNPs),与2号染色体上某个位点的甲酸盐水平相关,与PAS/LOV PROTEIN (PLP) 密切相关.
PLPA和PLPB拼接变种与维生素C缺陷2 (VTC2) 和VTC5.5共同定位. PLPA和PLPB与VTC2相互作用,蓝光减少了这种相互作用.
PLPB淘汰突变体显示出明显升高的甲酸盐水平,表明PLP在甲酸盐调节中的作用. 加密染色突变体也显示了改变的酸盐水平.

结论:

PAS/LOV PROTEIN (PLP) 在阿拉比多普西斯的高光适应过程中对提高酸盐水平至关重要.
该机制涉及PLP对VTC2的抑制作用,该VTC2在蓝光照明时会释放出来,这表明光线依赖的调节开关.
对于甲酸盐水平,存在复杂的环境控制层次,在高光应激时PLP占主导地位.

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