转录组分析揭示了AIS和肺腺癌的潜在生物机制
Rong-Xing Qin1, Yue Yang1, Jia-Feng Chen1
1Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China.
Frontiers in neurology
|June 2, 2023
概括
像CCNA2和CCNB1这样的枢纽基因是急性缺血性中风 (AIS) 和肺腺癌 (LUAD) 的关键. 它们的改变表达会影响细胞衰老,为这些疾病提供潜在的诊断和预后标志物.
科学领域:
- 在瘤学瘤学.
- 神经学 神经学
- 基因组学就是基因组学.
背景情况:
- 急性缺血性中风 (AIS) 和肺腺癌 (LUAD) 带来了高死亡率的全球健康挑战.
- 需要进一步阐明AIS和LUAD之间的复杂关系和因果关系.
研究的目的:
- 在AIS和LUAD中识别和功能性注释具有诊断潜力的关键基因 (枢纽基因).
- 研究这些已识别的枢纽基因的临床实用性和生物学作用.
主要方法:
- 利用了来自GEO和TCGA的转录组和单细胞数据.
- 通过PPI网络,ROC曲线和生存分析识别了差异表达基因 (DEG) 和枢纽基因.
- 评估了瘤细胞,免疫微环境和蛋白质水平中的基因表达,然后对潜在的治疗方法进行分子对接.
主要成果:
- 在AIS和LUAD之间确定了372个重叠的DEG,确定了CCNA2,CCNB1,CDKN2A和CDK1作为高度表达的枢纽基因.
- 枢纽基因与细胞周期,细胞衰老和HIF-1信号通路有关.
- 证实了这些枢纽基因的高诊断能力和与不良预后的关联;黄素被确定为潜在的治疗剂.
结论:
- 已识别的枢纽基因,特别是那些参与细胞衰老的基因,在AIS和LUAD病变发生过程中起着至关重要的作用.
- 这些枢纽基因代表了对AIS和LUAD的诊断和预后有前途的生物标志物.
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