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在心脏手术后早期心周液基马酶激活的影响
Brittany Butts1, Lee A Goeddel2, Jingyi Zheng3
1Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA, United States.
Frontiers in cardiovascular medicine
|June 2, 2023
概括
心脏手术后心周液中早期的化学酶激活预测了更长的住院时间. 细胞外囊泡将基马酶输送到心脏,造成损伤,这表明心脏手术后并发症的新型治疗点.
科学领域:
- 心血管生物学 心血管生物学
- 蛋白质酶生物化学 蛋白质酶生物化学
背景情况:
- 化学酶是一种破坏性的血清蛋白酶,通常被循环抑制剂中和.
- 了解化学酶在心脏手术后并发症中的作用至关重要.
研究的目的:
- 调查心周液 (PCF) 基酶激活和炎症标志物是否预测重症监护室 (ICU) 停留时间.
- 探索通过细胞外囊泡 (EVs) 向心脏输送化学酶的机制.
主要方法:
- 从心脏手术患者收集的PCF (在和离).
- 测定了基酶活性和32个炎症标志物.
- 注射PCF EVs含基马酶给大鼠,以评估心脏输送和损伤.
- 使用质谱和传输电子显微镜进行分析.
主要成果:
- 基酶活性和炎症标志物在式和非式组之间没有差异.
- PCF基酶活性,CXCL6和STS-PROM得分预测了ICU和住院总长度.
- 经证实,PCF EVs在老鼠中向心脏输送活性基酶,导致心肌细胞损伤.
- 在PCF中的蛋白酶抑制剂中和化酶,但EVs保护化酶免受中和.
结论:
- 早期的手术后PCF chymase激活与心脏损伤有关.
- 通过EVs传递的化学酶提供了一个受保护的机制,绕过循环抑制剂.
- 准EV介导的基酶递送可能会减轻心脏手术后的心脏损伤.
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