涉及母胎界面的巨细胞之间的细胞间通信可能是URSA的一个关键机制:从转录基因数据的新发现
Xiaoxuan Zhao1, Yuepeng Jiang2, Shiling Luo1
1Department of Traditional Chinese Medicine (TCM) Gynecology, Hangzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Hangzhou, China.
Frontiers in endocrinology
|June 2, 2023
概括
无法解释的复发性自发性流产 (URSA) 涉及巨细胞和果细胞之间的异常通信. 这项研究确定了改善URSA诊断的关键生物标志物 (FGF9,IL1R2,叶细胞的NID2;巨细胞的CFB,NID2,CXCL11).
科学领域:
- 生殖免疫学 生殖免疫学
- 蜂通讯 蜂通讯是通过蜂通讯进行的.
- 生物信息学是一种生物信息学.
背景情况:
- 无法解释的复发性自发性流产 (URSA) 在全球范围内构成了重大生殖挑战.
- 仍然不完全理解URSA的病因和发病原因,其中涉及巨细胞 (Mφ) 和叶 stromal 细胞 (DSCs) 或热囊细胞之间的异常细胞间通信.
- 现有的关于调节这种交叉通话的分子机制的知识是有限的.
研究的目的:
- 阐明URSA中Mφ和DSCs/trophoblasts之间细胞间通信的分子机制.
- 根据这些通信通道,确定URSA的诊断生物标志物.
主要方法:
- 综合方法结合了对基因表达综合 (GEO) 数据集 (decidual,胎盘,Mφ极化) 的生物信息学分析,蛋白质-蛋白质相互作用 (PPI) 网络构建,加权基因共同表达网络分析 (WGCNA) 和机器学习.
- 通过实时PCR,免疫丰富分析和免疫光共定位的验证.
主要成果:
- 在URSA患者的树叶和胎盘组织中识别了差异表达基因 (DEG),并在Mφ极化期间.
- 构建了PPI网络和确定了枢纽基因.
- 分别选和验证了来自DSC,胎盘和Mφ DEGs的29,43和22个分泌的蛋白质编码基因.
- 发现了三个具有诊断价值的DSC生物标志物 (FGF9,IL1R2,NID2) 和三个Mφ生物标志物 (CFB,NID2,CXCL11).
结论:
- 这项研究为URSA中母胎界面的巨细胞所涉及的细胞间通信的调节网络提供了新的见解.
- 已确定的特定生物标志物为URSA提供了创新的诊断和治疗潜力.
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