定量实时PCR循环值与培养物之间的关系,用于检测Streptococcus equi的亚种
J Scott Weese1, Matt Saab1, Allison Moore1
1Department of Pathobiology and Centre for Public Health and Zoonoses, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario NIG 2W1 (Weese); Diagnostic Services (Saab) and Department of Health Management (Saab, McClure), Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3; Animal Health and Welfare Branch, Ontario Ministry of Agriculture, Food and Rural Affairs, 5th Floor NW, 1 Stone Road West, Guelph, Ontario N1G 4Y2 (Moore); Animal Health Laboratory, University of Guelph, 419 Gordon Street, Guelph, Ontario N1G 2W1 (Cai).
定量PCR (qPCR) 可以检测到马中的Streptococcus equi,但只在一小部分阳性样本中发现了可活的细菌. 周期值 (CT) 为34.2,表明可培养的S. equi.的可能性更高.
科学领域:
- 兽医微生物学 兽医微生物学
- 马类传染病 马类传染病
- 分子诊断学 分子诊断
背景情况:
- 马杆菌 (Streptococcus equi equi) 亚种 (S. equi) 在马中引起呼吸道感染.
- 准确检测S. equi对于疾病管理和控制至关重要.
- 定量PCR (qPCR) 提供了一种快速的病原体检测方法.
研究的目的:
- 为了比较PCR和细菌培养用于S. equi检测的诊断性能.
- 为了建立一个可靠的周期值 (CT) 值来预测可培养的S. equi的存在.
主要方法:
- 158匹马的呼吸道样本使用qPCR进行了分析.
- 用qPCR检测阳性样本进行了细菌培养.
- 分析CT值,以确定培养能力的最佳断点.
主要成果:
- 在12%的样本中分离了S. equi.
- 随着CT值的增加,隔离可培养S. equi的可能性下降.
- CT值34.2被确定为最佳断点,平衡虚假阴性和虚假阳性.
结论:
- 少数qPCR阳性样本产生了可行的S. equi.
- qPCRCT值可以作为一种可培养S. equi.存在的代理值.
- CT值评估有助于对S. equi感染的风险评估.
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