对于使用脂肪进行聚氧甲酸生物生产的摇瓶和板种植的工作流程
Sebastian L Riedel1,2, Ewelina N Donicz1, Paula Ferré-Aparicio1
1Institute of Biotechnology, Chair of Bioprocess Engineering, Technische Universität Berlin, Ackerstraße 76 ACK 24, D-13355, Berlin, Germany.
Applied microbiology and biotechnology
|June 2, 2023
概括
废弃的动物脂肪可以用于生物生产. 优化的小瓶和井设计允许可再生的微生物培养,从这些疏水原料中产生大量的聚酸 (PHA).
科学领域:
- 生物技术是生物技术.
- 微生物培养 微生物培养
- 生物工艺工程 生物工艺工程
背景情况:
- 化学生物生产的自然资源有限.
- 废动物脂肪 (WAF) 提供了一个可持续的替代原料.
- 挑战包括WAF的高点和不溶性,由于薄膜/块的形成,阻碍了微生物的消耗.
研究的目的:
- 确定最佳的摇瓶和井设计,以培养像WAF这样的疏水原料的微生物.
- 使用WAF开发一种可复制的微生物生长和产品合成工作流程.
- 评估从WAF产生的多基酸盐 (PHA) 的产量.
主要方法:
- 研究了各种摇瓶和微波板设计.
- 在线测量溶解氧度.
- 在优化条件下使用WAF培养Ralstonia eutropha.
- 分析的细胞干重量 (CDW) 和聚基酸盐 (PHB) 含量.
主要成果:
- 气体质量转移在不同的摇瓶设计中存在显著差异.
- 高可重复性通过特定的混式瓶和井板设计实现.
- 通过使用混瓶,从1%的WAF中获得>6g/L的CDW与>80%的PHB.
- 在微波板中进行了改进的预乳化,产生了14g/L的CDW,其中70%的PHB来自3%的WAF.
结论:
- 特殊的水瓶和井设计,结合量身定制的条件,使得可用疏水原料进行可重复的栽培.
- 建立了一个工作流程,以快速选WAF作为PHA生产的原料.
- 该方法不仅适用于其他生物制品的PHA生产.
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