在海嗅觉器官中嗅觉受体表达的现场杂交分析
Shoko Nakamuta1, Masanori Mori2, Miho Ito2
1Laboratory of Veterinary Anatomy, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan.
Cell and tissue research
|June 2, 2023
概括
绿海使用其整个嗅觉器官来检测空气中的气味. 在水鼻中发现了I类气味受体 (OR),而II类OR广泛存在,表明可以检测空气和水中的气味.
科学领域:
- 嗅觉生物学 嗅觉生物学
- 海洋动物的感官系统
- 乌生理学 乌生理学
背景情况:
- 乌拥有双重的嗅觉系统,其中一个是空气中传递的气味的上腔表皮质 (UCE),另一个是水中传递的气味的下腔表皮质 (LCE).
- 气味受体 (ORs) 是嗅觉的关键,分为I类 (水溶性联结体) 和II类 (挥发性联结体).
- 在气味检测中UCE和LCE的确切作用仍然不清楚.
研究的目的:
- 研究绿色海 (Chelonia mydas) 幼的嗅觉器官中的I类和II类气味受体 (ORs) 的定位和表达模式.
- 阐明OR表达细胞在UCE和LCE中的功能分布.
- 了解不同OR类对海空气和水中嗅觉的贡献.
主要方法:
- 在现场杂交被用来分析I类和II类OR基因的空间表达.
- 这项研究的重点是绿色海Chelonia mydas的幼的嗅觉器官.
- 在UCE和LCE内确定OR表达细胞的定位.
主要成果:
- 在LCE中主要发现I类OR表达细胞,支持其在检测水中传递的气味物中的作用.
- II类OR表达细胞表现出更广泛的分布,存在于UCE和LCE中.
- 第二类ORs的广泛表达表明,整个嗅觉器官可能参与感知空气中的气味物.
结论:
- LCE主要涉及检测由水传播的气味物质,根据I类OR分布.
- UCE和LCE都有助于检测空气中的气味物质,由II类OR表达体现出来.
- 广泛的II类OR表达增强了海检测空气刺激的能力,这对于导航和食至关重要.
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