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基于CRISPR-Cas9的食品级基因编辑系统的构建及其在Lactococcus lactis NZ9000中的应用

Yangping Zhou¹, Fei Song^1,2, Hongru Yang^1,3

¹College of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China.

Biotechnology letters

|June 2, 2023

概括

一个新的CRISPR/Cas9基因编辑系统与红色/ET相结合,被开发用于乳球菌. 这种高效的食品级系统在没有抗生素或外源基因的情况下实现了91%的淘汰效率.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

聚类正规间隔短Palindromic重复相关蛋白9 (CRISPR/Cas9) 系统是一个强大的基因编辑工具.
乳酸细菌中现有的基因编辑方法,如乳球菌,往往是低效的,不是食品级的.
在乳酸细菌中需要高效的食品级基因编辑系统,用于工业应用.

研究的目的:

开发和优化一个高效的,食品级的基于CRISPR/Cas9的基因编辑系统,用于乳球菌.
与现有方法相比,提高Lactococcus lactis的淘汰效率.
建立一个避免使用抗生素和外源基因的系统.

主要方法:

一个重组的CRISPR/Cas9系统是通过将其与红色/ET同源重组组合而构建的.
该系统使用了乳球菌乳糖衍生等离子体pMG36e.
开发的系统应用于Lactococcus lactis中的基因编辑.

主要成果:

开发的重组系统在乳球菌中实现了91%的显著高淘汰效率.
该系统展示了高通量层次选的潜力.
在没有引入抗生素或外源基因的情况下,成功获得了基因编辑的Lactococcus lactis菌株.

结论:

一个高效和优化的基因编辑系统,用于乳酸细菌,特别是乳糖菌,已经成功构建.
开发的系统是食品级,高效,适合高通量应用.
这一进步为乳酸细菌的基因操纵提供了有价值的工具.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?基因编辑基因编辑基因组工程是基因组工程.乳酸脱酶的使用方法乳球球菌 (lactococcus lactis) 是一种乳球菌.

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