活产每次胚胎转移与下一代测序植入前遗传测试:对26,107个周期的分析
Papri Sarkar1, Erika P New1, Rachel G Sprague1,2
1Division of Reproductive Endocrinology and Infertility, University of South FL, Sarasota, FL, USA.
Systems biology in reproductive medicine
|June 2, 2023
概括
使用下一代测序 (NGS) 进行前植入基因测试 (PGT-A) 改善了每次移植的活产率,特别是在35岁及以上的女性中. 这种先进的PGT-A技术通过识别用于转移的euploid胚胎来提高试管婴儿的成功率.
科学领域:
- 生殖医学 生殖医学
- 遗传学 遗传学 是一个
- 在体外受精 (IVF)
背景情况:
- 前植入基因检测形积分病 (PGT-A) 技术已经显著发展.
- 目前的PGT-A实践通常涉及托菲克托皮活检和下一代测序 (NGS).
研究的目的:
- 评估PGT-A在当代试管婴儿中仅使用NGS的有效性.
- 分析PGT-A对每个胚胎移植 (ET) 的活产率 (LBR) 的影响.
主要方法:
- 来自Shady Grove生育 (SGF) 多中心实践的大型数据集的回顾性分析.
- 在2017年1月至2020年7月期间包括至少一个胚胎移植 (新鲜和/或冷) 的自主试管婴儿周期.
- 在接受PGT-A的研究组和不接受PGT-A的对照组之间的LBR比较.
主要成果:
- 根据年龄调整的每次转移的LBR在PGT-A组 (48.9%) 与非PGT-A组 (42.7%) 相比显著更高 (p < 0.001).
- 这一趋势适用于所有年龄段,除了接受冷单个胚胎移植的35岁以下妇女.
- 在卵巢储量下降的患者中,通过PGT-A进行的欧胚胎移植显示出更高的LBR (46.7%与26.7%,p < 0.001),并且在无法解释的不孕不育和子宫内膜异位症病例中观察到流产率降低.
结论:
- 基于NGS的PGT-A与每次转移的活产率有所改善有关.
- PGT-A的好处在35岁及以上的女性中尤为明显.
- 在特定的不孕症诊断中,PGT-A显示了降低流产率的潜力.
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