共同传播的广泛的转录后监管
Nannan Chen1, Yunpeng Zhang1, Emmanuel J Rivera-Rodriguez1
1Department of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USA.
Science advances
|June 2, 2023
概括
神经元可以释放多个神经递质,这个过程称为共传播. 微RNA miR-190积极抑制胆固醇共传输,其损失会扰乱Drosophila的睡眠模式.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 神经元通信传统上被认为是单一的,但现在已经认识到共传播 (释放多种神经活性物质).
- 调节单个神经元内神经递质的动态表达的机制在很大程度上仍未被探索.
研究的目的:
- 为了研究控制Drosophila中神经递质共传播的调节机制.
- 确定参与抑制或启用特定神经递质系统表达的分子参与者.
主要方法:
- 开发用于Drosophila中胆固醇,谷氨酸和GABAerg信号的转录和翻译记者.
- 使用微RNA (miRNA) 操纵和"翻译陷"策略来评估蛋白质表达和翻译.
- 在miRNA功能的基因改变后,对睡眠模式的分析.
主要成果:
- 许多Glutamatergic和GABAergic神经元转录胆固醇基因,但不会积累功能性胆固醇蛋白.
- 微RNAmiR-190积极抑制胆固醇转录表达,从而抑制共传播.
- 失去miR-190的功能导致胆固醇机械的表达,导致睡眠碎片化和减少.
- 神经元表现出胆固醇蛋白的短暂翻译,这表明共传抑制的活性调制.
结论:
- 通过miR-190进行后转录调节是限制快速神经递质共传的关键机制.
- 这种调节过程允许神经元输出的动态和可逆调整.
- 同传播的调节会影响睡眠等基本行为.
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