具有结构多糖层的同原细胞的植物细胞启发膜化
Yanglimin Ji1,2, Yiyang Lin3, Yan Qiao1,2
1Beijing National Laboratory for Molecular Sciences (BNLMS), Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Journal of the American Chemical Society
|June 2, 2023
概括
研究人员通过涂上多糖的液滴来制造合成原细胞, 模仿植物细胞壁. 这些稳定的原细胞可以隔离分子,沟通,甚至杀死细菌,促进合成细胞的发展.
科学领域:
- 仿生材料科学
- 合成生物学
- 体科学
背景情况:
- 分隔式体作为合成细胞发展的模型系统.
- 植物细胞壁激发了创建稳定,功能原细胞模型的策略.
研究的目的:
- 使用多糖涂层开发膜化协体作为原细胞模型.
- 研究这些工程原细胞的稳定性,功能性和仿生行为.
主要方法:
- 用刚性多糖化合物覆盖类似液体的微滴 (coacervates),形成一个保护膜.
- 评估体稳定性,生物分子封存和化学交换能力.
- 对细菌内部化和杀死 (大肠杆菌) 的刺激反应膜溶解的评估.
- 观察组织成类似组织的原细胞组.
主要成果:
- 膜化提供了体稳定性,防止聚合和凝聚.
- 聚糖膜促进了选择性的生物分子封存和交换.
- 由酶触发的膜溶解使大肠杆菌能够内化和杀死.
- 原细胞组织成结构化的组织,模仿细胞通信和新陈代谢.
结论:
- 具有多糖膜的原细胞的表面工程为先进的合成细胞模拟物创造了一个多功能平台.
- 这些膜化协同生物表现出复杂的类似细胞的行为,包括稳定性,选择性交换和有组织的组装.
- 开发的方法为构建功能合成细胞提供了一条途径,在各种生物领域具有潜在的应用.
相关概念视频
Cellulose and Pectic Polysaccharides
3.7K
Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
As a cell matures, its cell wall specializes according to its type. For example, the...
3.7K
Cell Adhesion in Plants
2.8K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
2.8K
Role of Microtubules in Cell Wall Deposition
2.5K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.5K
What are Membranes?
157.8K
A key characteristic of life is the ability to separate the external environment from the internal space. To do this, cells have evolved semi-permeable membranes that regulate the passage of biological molecules. Additionally, the cell membrane defines a cell’s shape and interactions with the external environment. Eukaryotic cell membranes also serve to compartmentalize the internal space into organelles, including the endomembrane structures of the nucleus, endoplasmic reticulum and...
157.8K
Biosynthesis of Polysaccharides
42
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
42
Chemistry of Carbohydrates
73.9K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
73.9K


