生物膜形成和与Mycoplasma synoviae中药物耐药性的相关性
Tianhao Kang1, Minghu Zhou1, Xiu Yan1
1School of life science and engineering, Foshan University, Foshan 528225, China.
Veterinary microbiology
|June 2, 2023
概括
来自中国的Mycoplasma synoviae菌株对抗生素敏感性降低,形成生物膜. 这些生物膜增加了对常见抗生素的耐药性,影响了感染性突炎的治疗.
科学领域:
- 兽医微生物学 兽医微生物学
- 禽类疾病 禽类疾病
- 细菌病原体的产生
背景情况:
- 的传染性突炎是由Mycoplasma synoviae引起的.
- 临床症状包括排泄性突膜和紧突炎.
研究的目的:
- 为了研究来自中国的Mycoplasma synoviae菌株.
- 描述它们对抗生素的敏感性和生物膜的形成.
- 评估生物膜对抗生素耐药性的影响.
主要方法:
- 隔离和鉴定Mycoplasma synoviae菌株.
- 用于K型和A型菌株鉴定的VlhA基因型定型.
- 抗生素敏感性测试对恩罗夫洛克萨,多西环素,蒂奥林和泰洛.
- 扫描电子显微镜用于生物膜结构分析.
- 确定最佳生物膜形成温度和与抗生素耐药性的相关性.
主要成果:
- 确定了29种K型和3种A型Mycoplasma synoviae菌株.
- 与类型菌株相比,所有孤立菌株对测试的抗生素的敏感性都降低了.
- 菌细胞共形成了具有明显形态的生物膜.
- 最佳的生物膜形成发生在33°C.
- 生物膜显著增强了对所有四种测试的抗生素的耐药性.
- 对恩罗夫洛克萨和生物膜生物质的最低生物膜抑制度之间观察到负相关性.
结论:
- 这项研究是首次报告Mycoplasma synoviae生物膜形成能力.
- 中国的Mycoplasma synoviae菌株因生物膜形成而表现出降低的抗生素敏感性和增强的耐药性.
- 这些发现为了解和对抗家禽中Mycoplasma synoviae感染提供了基础.
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