大型淡水湖泊生态系统中的细菌转录组中的功能基因转录变异:对生态系统和人类健康的影响
Javad Sadeghi1, Abdolrazagh Hashemi Shahraki1, Subba Rao Chaganti2
1Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, Canada.
Environmental research
|June 2, 2023
概括
淡水细菌群体在冬季保持活跃,表现出强烈的季节性变化. 大多数检测到的细菌处于休眠状态,健康风险的种类因季节而异.
科学领域:
- 环境微生物学环境微生物学
- 转录基因组学 (Metatranscriptomics) 是一种关于转录基因组学的研究.
- 淡水生态系统 淡水生态系统
背景情况:
- 对淡水细菌群体 (BC) 功能动态的理解有限,特别是在冬季的非开花条件下.
- 在水生生态系统健康和生物地球化学循环中BCs的重要性.
- 需要有关季节性变化和微生物活动/休眠状态的数据.
研究的目的:
- 通过metatranscriptomics研究淡水BCs跨季节的时间和空间功能变化.
- 评估细菌基因转录和活动在冬季与温暖的季节.
- 为了识别潜在的有害种群的季节性变化,并了解微生物休眠状态.
主要方法:
- 来自加拿大安大略省三个公共海的淡水样本的metatranscriptomic分析.
- 采样是在2019年冬季 (无冰),夏季和秋季进行的.
- 对细菌基因转录,KEGG通路和特定候选基因的分析.
主要成果:
- 观察到BC功能的强烈时间变化,空间变化最小.
- 即使在寒冷的冬季温度下,也检测到了显著的细菌基因活性 (89%的KEGG通路基因活跃).
- 只有32%的检测到的细菌属在转录上活跃,这表明广泛的休眠状态;健康风险类型的显著季节性变化.
结论:
- 淡水BCs在基因表达方面表现出适应性灵活性,以应对低温.
- 检测到的大量淡水细菌存在于休眠状态.
- 季节性变化对潜在病原性细菌的数量和活动产生重大影响,突出显示了对水安全和环境变化的影响.
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