CBFβ是由鱼病毒的春季病毒病引起的,并在斑马鱼中促进病毒复制
Ning Ji1, Wenji Huang1, Huifeng Dang1
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
Developmental and comparative immunology
|June 2, 2023
概括
斑马鱼核心结合因子β子单元 (CBFβ) 通过与病毒蛋白相互作用来帮助病毒感染. 这种转录因子促进病毒复制并抑制宿主.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 核心结合因子β子单元 (CBFβ) 是一种已知与病毒蛋白相互作用的转录因子.
- 了解CBFβ在宿主病毒相互作用中的作用对于开发抗病毒策略至关重要.
研究的目的:
- 为了识别和表征斑马鱼CBFβ同类物 (zfCBFβ).
- 研究zfCBFβ在抗病毒反应中对春季病毒性鱼病毒 (SVCV) 的作用.
主要方法:
- 对zfCBFβ的基因鉴定和序列分析.
- 在不同条件下的斑马鱼组织表达分析 (SVCV感染,多种I:C刺激).
- 在EPC细胞中进行过度表达研究,以评估对病毒标位和宿主基因表达的影响.
- 同免疫沉测试以确定相互作用的合作伙伴.
主要成果:
- 斑马鱼CBFβ (zfCBFβ) 非常类似于orthologs和构成性表达.
- 在SVCV感染和多I:C刺激时,zfcbfβ的表达在免疫组织中被诱导,但不是通过I型干扰素.
- 过度表达zfCBFβ增强SVCV复制,并调节宿主基因表达 (诱导tnfα,抑制isg15).
- zfCBFβ与SVCV蛋白 (SVCVP) 和宿主p53相互作用,增强其自身的稳定性.
结论:
- 斑马鱼CBFβ在促进病毒感染方面发挥作用,特别是SVCV.
- 病毒可能会准CBFβ以逃避或抑制宿主的抗病毒免疫反应.
- zfCBFβ与病毒和宿主蛋白相互作用,促进感染,并可能抑制抗病毒机制.
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