光RNA的双茎和分裂的结构变体 果体的光RNA
Jeremy Herrera-Gutierrez1, Steven J Burden1, Sarah E Kobernat1
1Biomolecular Sciences Graduate Programs, Boise State University, Boise, Idaho 83725, USA.
概括
研究人员开发了新的RNA Mango的aptamer变体,具有改进的光和设计灵活性. 这些双茎结构为RNA成像和检测RNA-RNA相互作用提供了增强的亮度和潜力.
科学领域:
- 分子生物学分子生物学
- 生物化学 生化学
- 生物技术是生物技术.
背景情况:
- 基亚体,如RNA Mango,对于RNA量化和跟踪有价值.
- 现有的RNA果体的单干结构限制了设计修改.
- G-四重复结构是Mango的RNA功能的关键.
研究的目的:
- 为了设计具有增强结构特征的新型RNA Mango 吸收体变体.
- 改进光特性,并扩大RNA果体的设计可能性.
- 探索RNA成像和检测RNA-RNA相互作用中的应用.
主要方法:
- 设计和合成了双茎RNA果体变体的变体.
- 进行光和度分析以量化结合和亮度.
- 引入并分析了第二个干的左侧区域的突变.
- 演示了分裂的功能,双分子果吸收体.
主要成果:
- 与原始相比,双茎RNA果变种的光度高达~75%的亮度.
- 第二个链子中的突变表明间接的连接体相互作用影响光.
- 第二根茎的链接器提供了合理设计和重新选择的潜力.
- 一个双分子果阿普塔默在体外成功制造和功能.
结论:
- 新的双茎RNA果体提供增强的光和更大的可设计性.
- 第二个干提供了一个平台,用于工程改进的aptamer性能.
- 双分子果体对检测RNA-RNA相互作用显示出前途.
- 这些进步扩大了RNA果体对各种生物应用的实用性.
相关概念视频
RNA-seq
10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
RNA Structure
71.7K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
71.7K
RNA Splicing
56.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.5K


