主体细胞线粒体和Coxiella burnetii之间的相互作用
Kai Qi Yek1, Diana Stojanovski2, Hayley J Newton3
1Department of Biochemistry and Pharmacology and the Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia; Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
International review of cell and molecular biology
|June 2, 2023
概括
一种Q热病原体Coxiella burnetii利用效应蛋白操纵宿主细胞线粒体. 了解这种相互作用揭示了对宿主-病原体动态和潜在治疗点的新见解.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病原体与宿主之间的相互作用
背景情况:
- 像Coxiella burnetii这样的细胞内细菌病原体必须操纵宿主细胞生物学才能生存和复制.
- C. burnetii 在修改后的真空体内复制,使用效应蛋白来劫持宿主细胞功能.
- 新出现的证据强调线粒体是C. burnetii效应物的关键目标.
研究的目的:
- 调查C. burnetii效应蛋白在向和操纵宿主细胞线粒体中的作用.
- 阐明C. burnetii感染期间线粒体功能 (如亡和蛋白质稳定) 如何受到影响.
- 了解线粒体蛋白质对宿主对C. burnetii的反应的贡献.
主要方法:
- 利用先进的omics方法和新技术.
- 采用各种实验方法来研究线粒体中的效应蛋白定位和功能.
- 在感染期间在线粒体水平上分析宿主-病原体相互作用.
主要成果:
- 线粒体被证实是C. burnetii效应蛋白的一个子集的目标.
- 线粒体功能,包括亡和蛋白质稳定,很可能受到这些效应者的影响.
- 线粒体蛋白质在宿主对C. burnetii的免疫反应中发挥作用.
结论:
- 研究线粒体中的宿主-病原体相互作用,为C. burnetii的发病提供了关键的见解.
- 新技术和omics方法使得对这种相互作用的高分辨率研究成为可能.
- 了解C. burnetii对线粒体的操纵,可以带来新的治疗策略.
相关概念视频
Mitochondria
14.0K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
14.0K
Mitochondrial Membranes
11.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
11.7K
Translocation of Proteins into the Mitochondria
3.2K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.2K
Electron Transport Chain: Complex III and IV
7.7K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.7K
Peroxisomes and Mitochondria
87.5K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
87.5K
Electron Transport Chain: Complex I and II
14.6K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
14.6K


